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Human A disintegrin and metalloproteinase with thrombospondin motifs 6 (ADAMTS6) ELISA Kit

Human A disintegrin and metalloproteinase with thrombospondin motifs 6 (ADAMTS6) ELISA Kit

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Specification

Product name Human A disintegrin and metalloproteinase with thrombospondin motifs 6 (ADAMTS6) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human A disintegrin and metalloproteinase with thrombospondin motifs 6 (ADAMTS6) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAMTS6 in samples. An antibody specific for ADAMTS6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAMTS6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ADAMTS6; ADAM-TS6; a disintegrin and metalloproteinase with thrombospondin motifs 6; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 6

Product Properties

Kit components • Human A disintegrin and metalloproteinase with thrombospondin motifs 6 microplate
• Human A disintegrin and metalloproteinase with thrombospondin motifs 6 standard
• Human A disintegrin and metalloproteinase with thrombospondin motifs 6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human A disintegrin and metalloproteinase with thrombospondin motifs 6 (ADAMTS6) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADAMTS6. No significant cross-reactivity or interference between Human ADAMTS6 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The predicted 860-amino acid ADAMTS6 protein has a domain structure characteristic of ADAMTSs. Beginning at the N terminus, it has a preproregion containing a potential furin cleavage site and a putative cysteine-switch, a catalytic domain containing a typical reprolysin-type zinc-binding signature and a 'met turn,' a disintegrin-like domain, a conserved thrombospondin module, a cysteine-rich domain, a spacer domain, and a second, less conserved thrombospondin module. The mature ADAMTS6 protein has 2 potential N-linked glycosylation sites. Northern blot analysis of several human tissues detected an approximately 8.5-kb ADAMTS6 transcript that was expressed at low levels in placenta and at barely detectable levels in a number of other tissues. Adamts6 was not detectably expressed during mouse embryo development.
Gene ID 11174
Alternative ADAMTS6; ADAM-TS6; a disintegrin and metalloproteinase with thrombospondin motifs 6; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 6
Accession Q9UKP5

Image & description

Fig.1. Human A disintegrin and metalloproteinase with thrombospondin motifs 6 (ADAMTS6) Standard Curve.

Fig.1. Human A disintegrin and metalloproteinase with thrombospondin motifs 6 (ADAMTS6) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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