Product name | CheKine™ Micro Lipid Peroxidation (MDA) Assay kit |
Applications notes | CheKine™ Micro Lipid Peroxidation (MDA) Assay Kit provides a convenient tool for detection of the malondialdehyde (MDA) present in a variety of samples. MDA, together with 4-hydroxynonenal (4-HNE), is a natural bi-product of lipid peroxidation and its quantification is generally used as marker for lipid peroxidation. The MDA in the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct,which can be easily quantified colorimetrically at OD 532 nm. Meanwhile, the absorbance at 600 nm was measured , and MDA was calculated by the difference between the absorbance at 532 nm and 600 nm (To avoid the interference of saccharose). |
Kit components | • Extraction Buffer • Reagent I • Reagent II |
Features & Benefits | • Determination of MDA in serum, plasma, urine, animal and plant tissues, cells, bacteria and other biological fluids. • Fast and convenient, measuring lipid peroxidation in a wide range of samples. |
Usage notes | • If not assayed immediately, samples can be stored at -80°C. |
Storage instructions | Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
Shipping | Gel pack with blue ice. |
Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background | Oxygen free radicals react on lipid unsaturated fatty acids to produce lipid peroxidation. The latter gradually breaks down into a series of complex compounds, including malondialdehyde (MDA). Lipid oxidation levels can be measured by detecting MDA levels. Lipid peroxidation may contribute to many diseases, including atherosclerosis, diabetes, and Alzheimer's disease. CheKine™ Micro Lipid Peroxidation (MDA) Assay kit provides a convenient tool for detection of the malondialdehyde (MDA) |
Fig.CheKine™ Micro Lipid Peroxidation (MDA) Assay kit
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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