| Product name | Mouse LIF protein, C-His tag |
| Sequence | Amino acid sequence derived from Mouse LIF protein (P09056) (Ser23-Phe202) was expressed, was expressed with a polyhistidine tag at the C-terminus. |
| Activity | Measured by its ability to induce proliferation in M-NFS-60 cells. The ED50 for this effect is 8.023 ng/mL. |
| Protein length | The recombinant Mouse LIF protein consists of 186 amino acids and has a predicted molecular mass of 20.5 KD. |
| Preparation method | E. coli |
| Purity | >98% as determined by SDS-PAGE |
| Alternative | Leukemia inhibitory factor; Differentiation-stimulating factor; lif; D factor |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Features & Benefits | Endotoxin: < 0.01 EU per µg of the protein as determined by the LAL method. |
| Molecular weight | 20.5 KD |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse LIF protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage instructions | Lyophilized Mouse LIF protein, C-His tagshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.17% HSA or BSA). Please prevent freeze-thaw cycles. |
| Shipping | The product is shipped at ambient temperature. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Leukemia inhibitory factor (LIF) is a 20 kD protein that belongs to the IL-6 receptor family, is a pleiotropic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. |
| Gene ID | 16878 |
| Alternative | Leukemia inhibitory factor; Differentiation-stimulating factor; lif; D factor |
| Accession | P09056 |
Fig. SDS-PAGE analysis of Mouse LIF protein, C-His tag.
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