| Product name | Mouse IL-33 protein |
| Sequence | Amino acid sequence derived from Mouse IL-33 isoform (Q8BVZ5) (Ser109-Ile266) was expressed. |
| Protein length | The recombinant Mouse IL-33 consists of 159 amino acids (N-Met) and has a predicted molecular mass of 17.7 kDa. The apparent molecular mass of the Mouse IL-33 is approximately 17 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| Preparation method | E. coli |
| Purity | >98 % as determined by SDS-PAGE |
| Alternative | C9orf26; DVS27; IL1F11; NF-HEV; NFEHEV; Interleukin-33; IL33 |
| Formulation | Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 1 mM DTT, 1 mM EDTA, pH 8.0. |
| Features & Benefits | Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method. |
| Molecular weight | 17.7 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse IL33 protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage instructions | Lyophilized Mouse IL-33 proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.9% HSA or BSA). Please prevent freeze-thaw cycles. |
| Shipping | The product is shipped at ambient temperature. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Interleukin 33 (IL-33), also known as DVS27 or NF-HEV (Nuclear Factor from High Endothelial enules), is a proinflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 protein is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyers's patches, and lymph nodes. IL-33 protein has transcriptional regulatory properties, and the researches suggested that IL-33 is a dual-function protein that might act both as a cytokine and as an intracellular nuclear factor. As a type 2 cytokines, IL-33 protein also play a pivotal role in helminthic infection and allergic disorders. |
| Gene ID | 77125 |
| Alternative | C9orf26; DVS27; IL1F11; NF-HEV; NFEHEV; Interleukin-33; IL33 |
| Accession | Q8BVZ5 |
Fig. SDS-PAGE analysis of Mouse IL-33 protein.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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