- Product name
Mouse IL-33 protein
Amino acid sequence derived from Mouse IL33 isoform (Q8BVZ5) (Ser109-Ile266) was expressed.
- Protein length
The recombinant Mouse IL33 consists of 159 amino acids (N-Met) and has a predicted molecular mass of 17.7 kDa. The apparent molecular mass of the Mouse IL33 is approximately 17 kDa in SDS-PAGE under reducing conditions due to glycosylation.
- Preparation method
>98 % as determined by SDS-PAGE
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 1 mM DTT, 1 mM EDTA, pH 8.0.
- Features & Benefits
Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.
- Molecular weight
- Usage notes
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse IL33 protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
- Storage instructions
Lyophilized Murine IL33 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
The product is shipped at ambient temperature.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Interleukin 33 (IL-33), also known as DVS27 or NF-HEV (Nuclear Factor from High Endothelial enules), is a proinflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 protein is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyers's patches, and lymph nodes. IL-33 protein has transcriptional regulatory properties, and the researches suggested that IL-33 is a dual-function protein that might act both as a cytokine and as an intracellular nuclear factor. As a type 2 cytokines, IL-33 protein also play a pivotal role in helminthic infection and allergic disorders.
- Gene ID
- Alternative names
C9orf26; DVS27; IL1F11; NF-HEV; NFEHEV; Interleukin-33; IL33
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Mouse IL-33 protein
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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
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Antibody application protocols
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- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
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- ♦ Handling and storage suggestion for peptides and protein
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Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
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