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CD4 Polyclonal Antibody

CD4 Polyclonal Antibody

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Specification

Product name CD4 Polyclonal Antibody
Immunogen Synthesized peptide derived from human CD4 around the non-phosphorylation site of S433
Host Rabbit
Reactivity Human, Mouse
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative CD4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD antigen CD4

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background CD4 encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. CD4 is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. CD4 molecule functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in CD4.
Gene ID 920
Alternative CD4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD antigen CD4
Others CD4 Polyclonal Antibody detects endogenous levels of CD4 protein.
Accession P01730
Observed Band(KD) 51

Image & description

Fig.1. Western Blot analysis of various cells using CD4 Polyclonal Antibody diluted at 1:500.

Fig.1. Western Blot analysis of various cells using CD4 Polyclonal Antibody diluted at 1:500.

Fig.2. Western Blot analysis of COLO205 cells using CD4 Polyclonal Antibody diluted at 1:500.

Fig.2. Western Blot analysis of COLO205 cells using CD4 Polyclonal Antibody diluted at 1:500.

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