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Human Glia-derived nexin (SERPINE2) ELISA Kit

Human Glia-derived nexin (SERPINE2) ELISA Kit

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Specification

Product name Human Glia-derived nexin (SERPINE2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Glia-derived nexin (SERPINE2) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINE2 in samples. An antibody specific for SERPINE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SERPINE2; GDN; PI7; PN1; PNI; OTTHUMP00000203766; glial-derived neurite promoting factor; glial-derived nexin 1; plasminogen activator inhibitor type 1; member 2; protease inhibitor 7 (protease nexin I); serine (o

Product Properties

Kit components • Human Glia-derived nexin microplate
• Human Glia-derived nexin standard
• Human Glia-derived nexin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Glia-derived nexin (SERPINE2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SERPINE2. No significant cross-reactivity or interference between Human SERPINE2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Protease nexin I is a 44-kD thrombin and urokinase inhibitor released by human foreskin fibroblasts. PN-I shares several features with antithrombin III , an abundant plasma thrombin inhibitor. Both PN-I and AT-III have high affinities for heparin, and heparin accelerates their rate of thrombin inhibition. In addition, the published sequence of 28 amino acids at the N-terminus of PN-I is identical to the N-terminal amino acid sequence of a glial-derived neurite promoting factor. Two forms, designated alpha and beta, were identified. These differed only by the insertion of 3 nucleotides into the coding sequence, and apparently arose by utilization of an alternative splice acceptor site in the PN1 gene. In the resulting proteins, alpha-PN-I contains an arginine residue at position 310, whereas beta-PN-I contains threonine-lysine residues.
Gene ID 5270
Alternative SERPINE2; GDN; PI7; PN1; PNI; OTTHUMP00000203766; glial-derived neurite promoting factor; glial-derived nexin 1; plasminogen activator inhibitor type 1; member 2; protease inhibitor 7 (protease nexin I); serine (o
Accession P07093

Image & description

Fig.1. Human Glia-derived nexin (SERPINE2) Standard Curve.

Fig.1. Human Glia-derived nexin (SERPINE2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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