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Mouse EGF protein

Mouse EGF protein

Views(5) Publications(0) Catalog no(PRP1957)
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Specification

Product name Mouse EGF protein
Activity Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.017 ng/mL. The specific activity of recombinant Mouse EGF is approximately >5.89 x 107IU/mg
Preparation method E. coli
Purity > 95% as determined by SDS-PAGE.
Molecular Formula The protein has a calculated MW of 6.98 kDa.
Alternative beta-urogastrone,EGF,epidermal growth factor, MEGF, HOMG4, URG, Urogastrone

Product Properties

Formulation The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Features & Benefits Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method.
Molecular weight The protein has a calculated MW of 6.98 kDa.
Usage notes Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Storage instructions Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution,  store at 2°C to 8°C for up to 1 week.  Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose  solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.EGF Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro.
Gene ID 13645
Alternative beta-urogastrone,EGF,epidermal growth factor, MEGF, HOMG4, URG, Urogastrone

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