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Human Zymogen granule protein 16 homolog B (ZG16B) ELISA Kit

Human Zymogen granule protein 16 homolog B (ZG16B) ELISA Kit

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Specification

Product name Human Zymogen granule protein 16 homolog B (ZG16B) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Zymogen granule protein 16 homolog B (ZG16B) ELISA Kit employs a two-site sandwich ELISA to quantitate ZG16B in samples. An antibody specific for ZG16B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZG16B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZG16B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZG16B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ZG16B; HRPE773; JCLN2; PAUF; PRO1567; jacalin-like lectin domain containing 2; pancreatic adenocarcinoma upregulated factor; zymogen granule protein 16 homolog B

Product Properties

Kit components • Human Zymogen granule protein 16 homolog B microplate
• Human Zymogen granule protein 16 homolog B standard
• Human Zymogen granule protein 16 homolog B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Zymogen granule protein 16 homolog B (ZG16B) ELISA Kit has high sensitivity and excellent specificity for detection of Human ZG16B. No significant cross-reactivity or interference between Human ZG16B and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ZG16b/PAUF has recently been found to play a role in gene regulation and cancer metastasis. However, the detailed functions of ZG16p and ZG16b remain to be clarified. ZG16p has a Jacalin-related β-prism fold, the first to be reported among mammalian lectins. The putative sugar-binding site of ZG16p is occupied by a glycerol molecule, mimicking the mannose bound to Jacalin-related mannose-binding-type plant lectins such as Banlec. ZG16b also has a β-prism fold, but some amino acid residues of the putative sugar-binding site differ from those of the mannose-type binding site suggesting altered preference. A positively charged patch, which may bind sulfated groups of the glycosaminoglycans, is located around the putative sugar-binding site of ZG16p and ZG16b. The sugar-binding site and the adjacent basic patch of ZG16p and ZG16b cooperatively form a functional glycosaminoglycan-binding site.
Gene ID 124220
Alternative ZG16B; HRPE773; JCLN2; PAUF; PRO1567; jacalin-like lectin domain containing 2; pancreatic adenocarcinoma upregulated factor; zymogen granule protein 16 homolog B
Accession Q96DA0

Image & description

Fig.1. Human Zymogen granule protein 16 homolog B (ZG16B) Standard Curve.

Fig.1. Human Zymogen granule protein 16 homolog B (ZG16B) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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