| Product name | Human Signal-regulatory protein beta-2 (SIRPB2) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Signal-regulatory protein beta-2 (SIRPB2) ELISA Kit employs a two-site sandwich ELISA to quantitate SIRPB2 in samples. An antibody specific for SIRPB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySIRPB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIRPB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIRPB2 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | SIRPB2; RP4-776F14.1; PTPN1L; PTPNS1L3; dJ776F14.2; protein tyrosine phosphatase; non-receptor type 1-like; protein tyrosine phosphatase; non-receptor type substrate 1-like 3 |
| Kit components | • Human Signal-regulatory protein beta-2 microplate • Human Signal-regulatory protein beta-2 standard • Human Signal-regulatory protein beta-2 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Signal-regulatory protein beta-2 (SIRPB2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SIRPB2. No significant cross-reactivity or interference between Human SIRPB2 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Signal-regulatory protein gamma is a protein encoded by this gene is a member of the signal-regulatory protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Alternatively spliced transcript variants encoding different isoforms have been described. The 354-amino acid protein shares approximately 75% sequence identity with SIRPB1. It possesses a putative signal sequence, a long extracellular domain, a transmembrane region, a short intracellular domain, and no catalytic domain. RT-PCR analysis detected expression of SIRPG at varying levels in all tissues tested. |
| Gene ID | 284759 |
| Alternative | SIRPB2; RP4-776F14.1; PTPN1L; PTPNS1L3; dJ776F14.2; protein tyrosine phosphatase; non-receptor type 1-like; protein tyrosine phosphatase; non-receptor type substrate 1-like 3 |
| Accession | Q5JXA9 |
Fig.1. Human Signal-regulatory protein beta-2 (SIRPB2) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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