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Human Y+L amino acid transporter 1 (SLC7A7) ELISA Kit

Human Y+L amino acid transporter 1 (SLC7A7) ELISA Kit

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Specification

Product name Human Y+L amino acid transporter 1 (SLC7A7) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Y+L amino acid transporter 1 (SLC7A7) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC7A7 in samples. An antibody specific for SLC7A7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC7A7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC7A7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC7A7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SLC7A7; LAT3; LPI; MOP-2; Y+LAT1; y+LAT-1; OTTHUMP00000164494; monocyte amino acid permease 2; solute carrier family 7 member 7; y(+)L-type amino acid transporter 1

Product Properties

Kit components • Human Y+L amino acid transporter 1 microplate
• Human Y+L amino acid transporter 1 standard
• Human Y+L amino acid transporter 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Y+L amino acid transporter 1 (SLC7A7) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC7A7. No significant cross-reactivity or interference between Human SLC7A7 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The SLC7A7 gene encodes the light subunit of a cationic amino acid transporter. Together with the heavy chain, 4F2hc (SLC3A2), the SLC7A7 protein forms a functional heterodimeric transporter on the basolateral cell membrane of epithelial cells that transfers lysine, arginine, and ornithine from the cell to the extracellular space The SLC7A7 gene encodes a deduced 511-residue protein with a molecular mass of 56 kD that contains 12 transmembrane domains. The protein sequence shows 81% homology to the opossum protein. Northern blot analysis identified a 2.4-kb mRNA transcript expressed in kidney, peripheral blood leukocytes, lung, placenta, spleen, and small intestine. SLC7A7 associated with SLC3A2 to form a functional 135-kD heterodimer linked by a disulfide bridge involving cysteine 109 of SLC3A2.
Gene ID 9056
Alternative SLC7A7; LAT3; LPI; MOP-2; Y+LAT1; y+LAT-1; OTTHUMP00000164494; monocyte amino acid permease 2; solute carrier family 7 member 7; y(+)L-type amino acid transporter 1
Accession Q9UM01

Image & description

Fig.1. Human Y+L amino acid transporter 1 (SLC7A7) Standard Curve.

Fig.1. Human Y+L amino acid transporter 1 (SLC7A7) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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