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IFKine™ Green Donkey Anti-Goat IgG

IFKine™ Green conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 518 nm. They are brighter than FITC, Cy2, and DyLight 488. IFKine™ Green conjugates are recommended for for all immunofluorescence procedures requiring a green-fluorescing dye.

Product Information

  • Product name

    IFKine™ Green Donkey Anti-Goat IgG

  • Immunogen

    Goat IgG whole molecule

  • Host


  • Reactivity


  • Applications

    FCM, ICC, IF

  • Application notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1000 dilutions for most fluorescent applications.

  • Clonality


  • Isotype

    Donkey IgG

  • Purification

    Affinity purified using solid phase Goat IgG (H&L) with finally > 95% purity based on SDS-PAGE

Product Properties

  • Formulation

    Liquid solution

  • Features & Benefits

    Abbkine IFKine™ is series of unique fluorence staining secondary antibodies with improved brightness, photostability and less nonspecific hybridization and background. The latest generation of IFKine fluorescent dyes ensure the best fluorescent performance, while it’s donkey host and other species of serum/IgG absorbed make IFKine™ secondary antibodies the ideal for use in fluorence staining, especially in fluorence multiple labeling.

  • Storage buffer

    Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol.

  • Storage instructions

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application.

  • Others

    The antibody reacts with whole molecule goat IgG. It also reacts with light chains of all other goat immunoglobulins. It's cross-reaction with human, mouse, rat, rabbit and guinea pig serum proteins has been specialized minimized to reduce non-specific hybrization. It has no reactivity on non-immunoglobulin serum proteins

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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through if you need more information or support.

  1. Electroacupuncture at ST-36 ameliorates DSS-induced acute colitis via regulating macrophage polarization induced by suppressing NLRP3/IL-1β and promoting Nrf2/HO-1

    Shuangning Song, Jing An, Yingli Li. Molecular Immunology.

  2. Electroacupuncture at ST-36 ameliorates DSS-induced acute colitis via regulating macrophage polarization induced by suppressing NLRP3/IL-1β and promoting Nrf2.

    S Song, J An, Y Li, S Liu - Molecular immunology, 2019.

  3. Protective role of p120‐catenin on mitochondria by inhibiting NLRP3 in ventilator‐induced lung injury

    G Liu, C Gu, M Liu, H Liu, D Wang. Journal of Cellular and Molecular Medicine,2019.

  4. Furin promotes dendritic morphogenesis and learning and memory in transgenic mice

    Zhu B, Zhao L, Luo D, et al. Cell Mol Life Sci, 2018.

  5. Neuropilin-2 promotes primary liver cancer progression by potentiating the activity of hepatic stellate cells

    Xu Z C, Shen H X, Chen C, et al. Oncol Lett, 2018, 15(2): 2245-2252.

  6. Polydatin Protects Diabetic Heart against Ischemia-Reperfusion Injury via Notch1/Hes2-Mediated Activation of Pten/Akt Signaling

    Yu L M, Li Z, Dong X, et al. Oxidative Medicine and Cellular Longevity, 2018 (2018).

  7. 17β-Estradiol on the Expression of G-Protein Coupled Estrogen Receptor (GPER/GPR30) Mitophagy, and the PI3K/Akt Signaling Pathway in ATDC5 Chondrocytes In Vitro

    Fan D X, Yang X H, Li Y N, et al. Med Sci Monit, 2018, 2; 24: 1936-1947.

  8. 17β-estradiol protects INS-1 insulinoma cells from mitophagy via G protein-coupled estrogen receptors and the PI3K/Akt signaling pathway

    Zhang L, Zhao Y and Guo L Int J Mol Med, 2018 , 41(5): 2839-2846.

  9. Clinical application of a microfluidic chip for immunocapture and quantification of circulating exosomes to assist breast cancer diagnosis and molecular classification

    Fang, Shimeng, et al. PloS one 12.4 (2017): e0175050.

  10. Expression of p96 in the inner ear hair cells in mice

    Chen,Zhiji,et al. Chinese Journal of Ophthalmology and Otorhinolaryngology,15.5(2015): 315-317.

  11. Down-regulation of the large conductance Ca2+-activated K+ channel expression in C57BL/6J cochlea.

    Pan C, Chu H, Lai Y, et al. Acta oto-laryngologica, 2016: 1-4.

  12. Interleukin-33 is released in spinal cord and suppresses experimental autoimmune encephalomyelitis in mice.

    Chen H, Sun Y, Lai L, et al. Neuroscience, 2015, 308: 157-168.

  13. Expression of FABP4, adipsin and adiponectin in Paneth cells is modulated by gut Lactobacillus.

    Su X, Yan H, Huang Y, et al. Scientific reports, 2015, 5.

Cat #: A24231

  • Size
  • Price
  • 100μl $70
  • 500μl $280

Contact information

Do not hesitate to contact us if you have any questions.

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  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

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