|Product name||IPKine™ HRP, Goat Anti-Mouse IgG HCS|
|Immunogen||Mouse IgG Fc Fragment|
|Applications||ELISA, IHC-P, WB|
|Applications notes||Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:10000), IHC-P (1:50-1:500), ELISA (1:500-1:10000).|
|Preparation method||Affinity purified using solid phase Mouse IgG(H+L) Fc Fragment with finally > 95% purity based on SDS-PAGE|
|Features & Benefits||50kD or 25kD protein detection on Western blots after Immunoprecipitation is often suffered from heavy or light chain blotting contamination). Abbkine's IPKine™ products solve these problems through less background noise, and enhanced accuracy). Their light chain/Fc fragment specific optimization will eliminate heavy/light chain interference, while serum proteins absorbed minimize cross-reactivity with immunoglobulins from other species which may be present on blots).|
|Storage buffer||Liquid in PBS, pH 7.4, containing 1% BSA as stablizer and 50% Glycerol.|
|Storage instructions||Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application.|
|Others||The antibody reacts with Fc portion of mouse IgG heavy chain, while it doesn’t react with the Fab portion of mouse IgG. It's cross-reaction with human, bovine and horse serum proteins has been specialized minimized, while it may cross-react with immunoglobulins from other species. It has no reactivity on non-immunoglobulin serum proteins|
Author： Jiang C, Ma N, Cao H Publication name：Microbiology Spectrum IF：9.043
Author：Z Wang, R Sun, G Wang Publication name：Redox biology IF：8.37
Author：X Liu, H Zhang, B Zhang Publication name：Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease IF：7.7
Author：Ding L, Zhang Z, Zhao C, Chen L, Chen Z, Zhang J, Liu Y, Nie Y, He Y, Liao K, Zhang X, Publication name：J Exp Clin Cancer Res IF：7.068
Author：Xiaoyu Tian, Pengli Zheng, Chenqian Zhou, et al Publication name：Autophagy IF：7.01
Author：Yan Q, Zhang B, Ling X, Zhu B, Mei S, Yang H, Zhang D, Huo J, Zhao Z, Publication name：Front Cell Dev Biol IF：5.201
Author：FJ Yan, X Wang, SE Wang Publication name：Biochemical Journal IF：4.05
Author：Zixiang Zhu, Pengfei Li, Fan Yang, et al Publication name：J Virol IF：4.02
Author：Shi R, Zhang J, Peng Z, et al Publication name：Int J Mol Med IF：2.91
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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