Product Information

• Product name

  IPKine™ HRP, Goat Anti-Mouse IgG HCS

• Immunogen

  Mouse IgG Fc Fragment

• Host

  Goat

• Reactivity

  Mouse

• Applications

  ELISA, IHC, WB

• Application notes

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000-1:100000), IHC (1:500-1:5000), ELISA (1:5000-1:100000).

• Clonality

  Polyclonal

• Isotype

  Goat IgG

• Purification

  Affinity purified using solid phase Mouse IgG(H+L) Fc Fragment with finally > 95% purity based on SDS-PAGE

• Conjugate

  HRP

Product Properties

• Formulation

  Liquid solution

• Features & Benefits

  50kD or 25kD protein detection on Western blots after Immunoprecipitation is often suffered from heavy or light chain blotting contamination). Abbkine's IPKine™ products solve these problems through less background noise, and enhanced accuracy). Their light chain/Fc fragment specific optimization will eliminate heavy/light chain interference, while serum proteins absorbed minimize cross-reactivity with immunoglobulins from other species which may be present on blots).

• Storage buffer

  Liquid in PBS, pH 7.4, containing 1% BSA as stablizer and 50% Glycerol.

• Storage instructions

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Shipping

  Gel pack with blue ice.

• Precautions

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

• Background

  Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application.

• Others

  The antibody reacts with Fc portion of mouse IgG heavy chain, while it doesn’t react with the Fab portion of mouse IgG. It's cross-reaction with human, bovine and horse serum proteins has been specialized minimized, while it may cross-react with immunoglobulins from other species. It has no reactivity on non-immunoglobulin serum proteins