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Human Phosphatidylethanolamine N-methyltransferase (PEMT) ELISA Kit

Human Phosphatidylethanolamine N-methyltransferase (PEMT) ELISA Kit

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Specification

Product name Human Phosphatidylethanolamine N-methyltransferase (PEMT) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Phosphatidylethanolamine N-methyltransferase (PEMT) ELISA Kit employs a two-site sandwich ELISA to quantitate PEMT in samples. An antibody specific for PEMT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPEMT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PEMT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PEMT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PEMT; MGC2483; PEAMT; PEMPT; PEMT2; PNMT; OTTHUMP00000065537

Product Properties

Kit components • Human Phosphatidylethanolamine N-methyltransferase microplate
• Human Phosphatidylethanolamine N-methyltransferase standard
• Human Phosphatidylethanolamine N-methyltransferase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Phosphatidylethanolamine N-methyltransferase (PEMT) ELISA Kit has high sensitivity and excellent specificity for detection of Human PEMT. No significant cross-reactivity or interference between Human PEMT and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Phosphatidylethanolamine N-methyltransferase (abbreviated PEMT) is a transferase enzyme (EC 2.1.1.17) which converts phosphatidylethanolamine (PE) to phosphatidylcholine (PC) in the liver. In humans it is encoded by the PEMT gene within the Smith-Magenis syndrome region on chromosome 17.While the CDP-choline pathway, in which choline obtained either by dietary consumption or by metabolism of choline-containing lipids is converted to PC, accounts for approximately 70% of PC biosynthesis in the liver, the PEMT pathway has been shown to have played a critical evolutionary role in providing PC during times of starvation. Furthermore, PC made via PEMT plays a wide range of physiological roles, utilized in choline synthesis, hepatocyte membrane structure, bile secretion, and very-low-density lipoprotein (VLDL) secretion.
Gene ID 10400
Alternative PEMT; MGC2483; PEAMT; PEMPT; PEMT2; PNMT; OTTHUMP00000065537
Accession Q9UBM1

Image & description

Fig.1. Human Phosphatidylethanolamine N-methyltransferase (PEMT) Standard Curve.

Fig.1. Human Phosphatidylethanolamine N-methyltransferase (PEMT) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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