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CD13 Polyclonal Antibody

CD13 Polyclonal Antibody

Views(71) Publications(0) Catalog no(ABP53310)
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Specification

Product name CD13 Polyclonal Antibody
Immunogen Synthesized peptide derived from the C-terminal region of human CD13
Host Rabbit
Reactivity Human
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative ANPEP; APN; CD13; PEPN; Aminopeptidase N; AP-N; hAPN; Alanyl aminopeptidase; Aminopeptidase M; AP-M; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; gp150; CD13

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma.
Gene ID 290
Alternative ANPEP; APN; CD13; PEPN; Aminopeptidase N; AP-N; hAPN; Alanyl aminopeptidase; Aminopeptidase M; AP-M; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; gp150; CD13
Others CD13 Polyclonal Antibody detects endogenous levels of CD13 protein.
Accession P15144
Observed Band(KD) 110

Image & description

Fig.1. Western Blot analysis of Mouse brain cells using CD13 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of Mouse brain cells using CD13 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.

Fig.4. Western Blot analysis of Mouse-liver Mouse-heart Mouse-kidney Mouse-spleen Mouse-brain Mouse-heart using CD13 Polyclonal Antibody diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.4. Western Blot analysis of Mouse-liver Mouse-heart Mouse-kidney Mouse-spleen Mouse-brain Mouse-heart using CD13 Polyclonal Antibody diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

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