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Human Secreted phospholipase A2 (sPLA2) ELISA Kit

Human Secreted phospholipase A2 (sPLA2) ELISA Kit

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Specification

Product name Human Secreted phospholipase A2 (sPLA2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Secreted phospholipase A2 (sPLA2) ELISA Kit employs a two-site sandwich ELISA to quantitate sPLA2 in samples. An antibody specific for sPLA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anysPLA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sPLA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sPLA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative sPLA2

Product Properties

Kit components • Human Secreted phospholipase A2 microplate
• Human Secreted phospholipase A2 standard
• Human Secreted phospholipase A2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Secreted phospholipase A2 (sPLA2) ELISA Kit has high sensitivity and excellent specificity for detection of Human sPLA2. No significant cross-reactivity or interference between Human sPLA2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Phospholipases A2 (PLA2s) hydrolyze the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids. PLA2G2A belongs to PLA2 group II, which consists of extracellular enzymes with low molecular masses that require calcium ions for catalysis . The deduced protein contains a 20-amino acid signal sequence, followed by a 124-amino acid mature peptide that has a calculated molecular mass of about 14 kD. The protein has characteristics of the type II class of PLA2s, including conserved catalytic residues and a Ca(2+)-binding loop. Northern blot analysis detected a 0.8-kb transcript in inflamed human synovial tissue and in peritoneal exudate cells from a peritonitis patient, but not in 2 activated human promyelocytic cell lines.
Alternative sPLA2

Image & description

Fig.1. Human Secreted phospholipase A2 (sPLA2) Standard Curve.

Fig.1. Human Secreted phospholipase A2 (sPLA2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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