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EliKine™ Human Alpha Fetoprotein (AFP) ELISA Kit

EliKine™ Human Alpha Fetoprotein (AFP) ELISA Kit

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Product name EliKine™ Human Alpha Fetoprotein (AFP) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes EliKine™ Human Alpha Fetoprotein (AFP) ELISA Kit employs a double antibody sandwich method to quantitate Human AFP in samples. An antibody specific for Human AFP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Human AFP present is bound by the immobilized antibody. After removing any unbound substances, an HRP-conjugated antibody specific for Human AFP is added to the wells. After washing, remove any unbound HRP-conjugated antibody reagent, adding HRP Substrate (TMB), TMB turns blue under the catalysis of HRP, and turns yellow after adding stop solution. Measure the OD value with a microplate reader at 450 nm wavelength. The Human Alpha Fetoprotein (AFP) concentration is proportional to the OD450 nm value.
Detection method Colorimetric
SampleType Cell culture supernatants, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3.5 hours. It depends on the experience of the operation person.

Product Properties

Kit components
• Human AFP Microplate
• Human AFP Standard (lyophilized)
• Sample Diluent (5×)
• Assay Buffer (5×)
• HRP-conjugated Human AFP Detect Antibody (100×)
• HRP Substrate(TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers
Features & Benefits EliKine™ Human Alpha Fetoprotein (AFP) ELISA Kit has high sensitivity and excellent specificity for detection of Human Alpha Fetoprotein (AFP), No significant cross-reactivity or interference between Human Alpha Fetoprotein (AFP) and analogues was observed.
Calibration range 0.625-40 ng/mL
Limit of detection 0.313 ng/mL
Usage notes
• 1. If Sample Diluent (5×) and Assay Buffer (5×) appears to turn yellow or a small amount of precipitation, etc., it is caused by the serum contained in the reagent. Please centrifuge to remove the precipitate, which will not affect normal use.
• 2. Do not mix or substitute reagents with those from other lots or sources.
• 3. To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent.
• 4. To ensure accurate results, proper adhesion of plate covers during incubation steps is necessary.
• 5. Stop Solution has certain Corrosive. Please take protective measures when operating.
Storage instructions Stored at 4℃ for 12 months.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Alpha-fetoprotein (AFP) is a highly specific and sensitive tumor marker for primary liver cancer. Its Cutoff value is generally 20 μg/L, when more than 500 μg/L or content rising, more certain significance.

Image & description

Fig.1. EliKine™ Human Alpha Fetoprotein (AFP) ELISA Kit

Fig.1. EliKine™ Human Alpha Fetoprotein (AFP) ELISA Kit

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