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Human Reticulon-4 receptor (RTN4R) ELISA Kit

Human Reticulon-4 receptor (RTN4R) ELISA Kit

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Specification

Product name Human Reticulon-4 receptor (RTN4R) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Reticulon-4 receptor (RTN4R) ELISA Kit employs a two-site sandwich ELISA to quantitate RTN4R in samples. An antibody specific for RTN4R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRTN4R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RTN4R is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RTN4R bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative RTN4R; NGR; NOGOR; Nogo-66 receptor; OTTHUMP00000197866; UNQ330/PRO526; nogo receptor

Product Properties

Kit components • Human Reticulon-4 receptor microplate
• Human Reticulon-4 receptor standard
• Human Reticulon-4 receptor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Reticulon-4 receptor (RTN4R) ELISA Kit has high sensitivity and excellent specificity for detection of Human RTN4R. No significant cross-reactivity or interference between Human RTN4R and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Reticulon 4 receptor is the receptor for reticulon 4, oligodendrocytemyelin glycoprotein and myelin-associated glycoprotein. This receptor mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult central nervous system. The Nogo-66 Receptor (NgR) is a high affinity binding receptor for a region of Nogo, a myelin associated protein that inhibits axon outgrowth. NgR was identified by Strittmatter and colleagues using an expression cloning strategy.NgR is implicated in neuronal plasticity and regeneration. Its relative importance in mediating myelin inhibition in vitro and in vivo is currently under intense investigation, since this protein might be a good drug target for treatment of various neurological conditions such as spinal cord injury and stroke.
Gene ID 65078
Alternative RTN4R; NGR; NOGOR; Nogo-66 receptor; OTTHUMP00000197866; UNQ330/PRO526; nogo receptor
Accession Q9BZR6

Image & description

Fig.1. Human Reticulon-4 receptor (RTN4R) Standard Curve.

Fig.1. Human Reticulon-4 receptor (RTN4R) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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