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Human Tomoregulin-1 (TMEFF1) ELISA Kit

Human Tomoregulin-1 (TMEFF1) ELISA Kit

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Specification

Product name Human Tomoregulin-1 (TMEFF1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Tomoregulin-1 (TMEFF1) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEFF1 in samples. An antibody specific for TMEFF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEFF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEFF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEFF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TMEFF1; C9orf2; H7365; tomoregulin-1

Product Properties

Kit components • Human Tomoregulin-1 microplate
• Human Tomoregulin-1 standard
• Human Tomoregulin-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Tomoregulin-1 (TMEFF1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMEFF1. No significant cross-reactivity or interference between Human TMEFF1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background EGF-like domain is an evolutionary conserved protein domain, of about thirty to forty amino-acid residues long, which was found in a large number of mostly animal proteins.Eib and Martens (1996) cloned a novel Xenopus gene, 7365, that is enriched in neuroendocrine tissues. The predicted protein contains a signal peptide, a transmembrane domain, 2 follistatin modules, and an EGF-like domain, a cysteine-rich region first identified in epidermal growth factor. Eib et al. (1998) stated that the unique EGF-like domain and transmembrane and cytoplasmic regions of the human homolog are nearly identical to those found in the mouse and frog homologs.Eib et al. (1998) used somatic cell hybrids and fluorescence in situ hybridization to map the TMEFF1 gene to human chromosome 9q31.
Gene ID 100526694
Alternative TMEFF1; C9orf2; H7365; tomoregulin-1
Accession Q8IYR6

Image & description

Fig.1. Human Tomoregulin-1 (TMEFF1) Standard Curve.

Fig.1. Human Tomoregulin-1 (TMEFF1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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