| Product name | CheKine™ Micro Isocitrate Dehydrogenase Cytoplasmic (ICDHc) Activity Assay Kit |
| SampleType | Animal and plant tissues, cells or bacteria, serum or plasma |
| Alternative | Isocitrate Dehydrogenase Cytoplasmic (ICDHc) |
| Kit components | •Extraction Buffer •Reagent I •Reagent Ⅱ •Reagent Ⅲ |
| Features & Benefits | The verified samples are complete in type and easy to operate． |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples. |
| Storage instructions | Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Cytoplasmic isocitrate dehydrogenase (ICDHc, EC 1.1.1.42) is widely distributed in animals, plants, microorganisms, and cultured cells. It catalyzes the decarboxylation and dehydrogenation of isocitrate to produce α-ketoglutarate, while simultaneously reducing NADP+ to NADPH. ICDHc serves as another significant source of NADPH in the cytoplasm, apart from the pentose phosphate pathway, and its activity often undergoes notable changes under stress conditions. CheKine™ Micro Isocitrate Dehydrogenase Cytoplasmic (ICDHc) Activity Assay Kit is capable of detecting cytosolic isocitrate dehydrogenase (ICDHc) activity in animal and plant tissues, cells, or bacteria, as well as in serum (plasma). The principle behind this involves utilizing ICDHc's catalytic action in reducing NADP+ to NADPH, with the subsequent increase in NADPH concentration being measured at a wavelength of 340 nm. |
| Alternative | Isocitrate Dehydrogenase Cytoplasmic (ICDHc) |
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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