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Human Pyridoxal/pyridoxine vitamin B6-kinase (PDXK) ELISA Kit

Human Pyridoxal/pyridoxine vitamin B6-kinase (PDXK) ELISA Kit

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Specification

Product name Human Pyridoxal/pyridoxine vitamin B6-kinase (PDXK) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Pyridoxal/pyridoxine vitamin B6-kinase (PDXK) ELISA Kit employs a two-site sandwich ELISA to quantitate PDXK in samples. An antibody specific for PDXK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDXK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDXK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDXK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PDXK; C21orf124; C21orf97; DKFZp566A071; FLJ31940; FLJ37311; MGC15873; MGC31754; MGC52346; PKH; PNK; PRED79; pyridoxal kinase; pyridoxamine kinase; pyridoxine kinase; vitamin B6 kinase

Product Properties

Kit components • Human Pyridoxal/pyridoxine vitamin B6-kinase microplate
• Human Pyridoxal/pyridoxine vitamin B6-kinase standard
• Human Pyridoxal/pyridoxine vitamin B6-kinase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Pyridoxal/pyridoxine vitamin B6-kinase (PDXK) ELISA Kit has high sensitivity and excellent specificity for detection of Human PDXK. No significant cross-reactivity or interference between Human PDXK and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Pyridoxal (pyridoxine, vitamin B6) kinase is also known as PDXK. The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. Hanna et al. (1997) cloned the cDNA encoding PDXK, which they referred to as PKH. The PDXK gene encodes a 312-amino acid polypeptide, and expression of the cDNA revealed pyridoxal kinase activity. Northern blot analysis revealed that a major 1.5-kb PDXK transcript was expressed in all tissues tested.
Gene ID 8566
Alternative PDXK; C21orf124; C21orf97; DKFZp566A071; FLJ31940; FLJ37311; MGC15873; MGC31754; MGC52346; PKH; PNK; PRED79; pyridoxal kinase; pyridoxamine kinase; pyridoxine kinase; vitamin B6 kinase
Accession O00764

Image & description

Fig.1. Human Pyridoxal/pyridoxine vitamin B6-kinase (PDXK) Standard Curve.

Fig.1. Human Pyridoxal/pyridoxine vitamin B6-kinase (PDXK) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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