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Human Piwi-like protein 1 (PIWIL1) ELISA Kit

Human Piwi-like protein 1 (PIWIL1) ELISA Kit

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Specification

Product name Human Piwi-like protein 1 (PIWIL1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Piwi-like protein 1 (PIWIL1) ELISA Kit employs a two-site sandwich ELISA to quantitate PIWIL1 in samples. An antibody specific for PIWIL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIWIL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIWIL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIWIL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PIWIL1; HIWI; MIWI; PIWI; piwi homolog; piwi-like 1

Product Properties

Kit components • Human Piwi-like protein 1 microplate
• Human Piwi-like protein 1 standard
• Human Piwi-like protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Piwi-like protein 1 (PIWIL1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PIWIL1. No significant cross-reactivity or interference between Human PIWIL1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PIWIL1 encodes a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. The encoded protein may play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells. Miwi associated with both piRNAs and mRNAs in cytosolic ribonucleoprotein and polysome fractions of mouse testicular extract. As polysomes increased in early spermiogenesis, Miwi levels increased in polysome fractions. Moreover, Miwi associated with the mRNA cap-binding complex.
Gene ID 9271
Alternative PIWIL1; HIWI; MIWI; PIWI; piwi homolog; piwi-like 1
Accession Q96J94

Image & description

Fig.1. Human Piwi-like protein 1 (PIWIL1) Standard Curve.

Fig.1. Human Piwi-like protein 1 (PIWIL1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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