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Mouse SUMO-conjugating enzyme UBC9 (UBE2I) ELISA Kit

Mouse SUMO-conjugating enzyme UBC9 (UBE2I) ELISA Kit

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Specification

Product name Mouse SUMO-conjugating enzyme UBC9 (UBE2I) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse SUMO-conjugating enzyme UBC9 (UBE2I) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE2I in samples. An antibody specific for UBE2I has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBE2I present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBE2I is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE2I bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative UBE2I; C358B7.1; P18; UBC9; OTTHUMP00000198126; OTTHUMP00000198127; SUMO-1-protein ligase; ubiquitin carrier protein; ubiquitin conjugating enzyme 9; ubiquitin-conjugating enzyme E2I; ubiquitin-conjugating enzym

Product Properties

Kit components • Mouse SUMO-conjugating enzyme UBC9 microplate
• Mouse SUMO-conjugating enzyme UBC9 standard
• Mouse SUMO-conjugating enzyme UBC9 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse SUMO-conjugating enzyme UBC9 (UBE2I) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse UBE2I. No significant cross-reactivity or interference between Mouse UBE2I and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2I encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.The protein has a predicted sequence of 158 amino acids and can support the growth of yeast ubc9 temperature-sensitive mutants at non-permissive temperatures, indicating that their gene is a functional homolog of yeast ubc9.
Gene ID 22196
Alternative UBE2I; C358B7.1; P18; UBC9; OTTHUMP00000198126; OTTHUMP00000198127; SUMO-1-protein ligase; ubiquitin carrier protein; ubiquitin conjugating enzyme 9; ubiquitin-conjugating enzyme E2I; ubiquitin-conjugating enzym
Accession P63280

Image & description

Fig.1. Mouse SUMO-conjugating enzyme UBC9 (UBE2I) Standard Curve.

Fig.1. Mouse SUMO-conjugating enzyme UBC9 (UBE2I) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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