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Human Cell cycle control protein 50A (TMEM30A) ELISA Kit

Human Cell cycle control protein 50A (TMEM30A) ELISA Kit

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Specification

Product name Human Cell cycle control protein 50A (TMEM30A) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Cell cycle control protein 50A (TMEM30A) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM30A in samples. An antibody specific for TMEM30A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM30A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM30A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM30A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TMEM30A; C6orf67; CDC50A; FLJ10856;

Product Properties

Kit components • Human Cell cycle control protein 50A microplate
• Human Cell cycle control protein 50A standard
• Human Cell cycle control protein 50A detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Cell cycle control protein 50A (TMEM30A) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMEM30A. No significant cross-reactivity or interference between Human TMEM30A and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Antitumor alkylphospholipids initiate apoptosis in transformed HL-60 and Jurkat cells while sparing their progenitors. 1-O-Alkyl-2-carboxymethyl-sn-glycero-3-phosphocholine (Edelfosine) like other short-chained phospholipids--inflammatory platelet-activating factor (PAF) and apoptotic oxidatively truncated phospholipids--are proposed to have intracellular sites of action, yet a conduit for these choline phospholipids into mammalian cells is undefined. Edelfosine is also accumulated by Saccharomyces cerevisiae in a process requiring the membrane protein Lem3p, and the human genome contains a Lem3p homolog TMEM30a. Import of choline phospholipids into S. cerevisiae ΔLem3 is partially reconstituted by human TMEM30a and by Lem3p-TMEM30a chimeras, showing the proteins are orthologous.
Gene ID 55754
Alternative TMEM30A; C6orf67; CDC50A; FLJ10856;
Accession Q9NV96

Image & description

Fig.1. Human Cell cycle control protein 50A (TMEM30A) Standard Curve.

Fig.1. Human Cell cycle control protein 50A (TMEM30A) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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