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Human Peptide chain release factor 1, mitochondrial (MTRF1) ELISA Kit

Human Peptide chain release factor 1, mitochondrial (MTRF1) ELISA Kit

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Specification

Product name Human Peptide chain release factor 1, mitochondrial (MTRF1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Peptide chain release factor 1, mitochondrial (MTRF1) ELISA Kit employs a two-site sandwich ELISA to quantitate MTRF1 in samples. An antibody specific for MTRF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTRF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTRF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTRF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MTRF1; RP11-74J13.3; MGC47721; MRF1; MTTRF1; RF1; OTTHUMP00000018316; mitochontrial peptide chain release factor 1

Product Properties

Kit components • Human Peptide chain release factor 1, mitochondrial microplate
• Human Peptide chain release factor 1, mitochondrial standard
• Human Peptide chain release factor 1, mitochondrial detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Peptide chain release factor 1, mitochondrial (MTRF1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MTRF1. No significant cross-reactivity or interference between Human MTRF1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Mitochondrial translation release factor 1 encoded by MTRF1 was determined by in silico methods to be a mitochondrial protein with similarity to the peptide chain release factors (RFs) discovered in bacteria and yeast. The peptide chain release factors direct the termination of translation in response to the peptide chain termination codons. Initially thought to have a role in the termination of mitochondria protein synthesis, a recent publication found no mitochondrial translation release functionality. Multiple alternatively spliced transcript variants have been suggested by mRNA and EST data; however, their full-length natures are not clear.
Gene ID 9617
Alternative MTRF1; RP11-74J13.3; MGC47721; MRF1; MTTRF1; RF1; OTTHUMP00000018316; mitochontrial peptide chain release factor 1
Accession O75570

Image & description

Fig.1. Human Peptide chain release factor 1, mitochondrial (MTRF1) Standard Curve.

Fig.1. Human Peptide chain release factor 1, mitochondrial (MTRF1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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