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Human 14-3-3 protein zeta/delta (YWHAZ) ELISA Kit

Human 14-3-3 protein zeta/delta (YWHAZ) ELISA Kit

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Specification

Product name Human 14-3-3 protein zeta/delta (YWHAZ) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human 14-3-3 protein zeta/delta (YWHAZ) ELISA Kit employs a two-site sandwich ELISA to quantitate YWHAZ in samples. An antibody specific for YWHAZ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyYWHAZ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YWHAZ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YWHAZ bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative YWHAZ; 14-3-3-zeta; KCIP-1; MGC111427; MGC126532; MGC138156; 14-3-3 protein/cytosolic phospholipase A2; 14-3-3 zeta; OTTHUMP00000165851; OTTHUMP00000165858; phospholipase A2; protein kinase C inhibitor protein-

Product Properties

Kit components • Human 14-3-3 protein zeta/delta microplate
• Human 14-3-3 protein zeta/delta standard
• Human 14-3-3 protein zeta/delta detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human 14-3-3 protein zeta/delta (YWHAZ) ELISA Kit has high sensitivity and excellent specificity for detection of Human YWHAZ. No significant cross-reactivity or interference between Human YWHAZ and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background YWHAz belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The deduced 245-amino acid protein shares high sequence homology with other 14-3-3 subtypes. The zeta mRNA was widely expressed in various gray matter brain regions, including the neocortex, hippocampus, caudate-putamen, thalamus, cerebellar cortex, and several brain stem nuclei. A human protein with phospholipase A2 activity was shown to be the zeta subtype of the 14-3-3 protein
Gene ID 7534
Alternative YWHAZ; 14-3-3-zeta; KCIP-1; MGC111427; MGC126532; MGC138156; 14-3-3 protein/cytosolic phospholipase A2; 14-3-3 zeta; OTTHUMP00000165851; OTTHUMP00000165858; phospholipase A2; protein kinase C inhibitor protein-
Accession P63104

Image & description

Fig.1. Human 14-3-3 protein zeta/delta (YWHAZ) Standard Curve.

Fig.1. Human 14-3-3 protein zeta/delta (YWHAZ) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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