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Human Kallikrein-2 (KLK2) ELISA Kit

Human Kallikrein-2 (KLK2) ELISA Kit

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Product name Human Kallikrein-2 (KLK2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Kallikrein-2 (KLK2) ELISA Kit employs a two-site sandwich ELISA to quantitate KLK2 in samples. An antibody specific for KLK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative KLK2; KLK2A2; MGC12201; hK2; glandular kallikrein 2; kallikrein 2; prostatic

Product Properties

Kit components • Human Kallikrein-2 microplate
• Human Kallikrein-2 standard
• Human Kallikrein-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Kallikrein-2 (KLK2) ELISA Kit has high sensitivity and excellent specificity for detection of Human KLK2. No significant cross-reactivity or interference between Human KLK2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Kallikrein-2 is a protein encoded by the KLK2 gene.The glandular kallikreins are a distinct group of serine proteases with molecular masses of 25,000 to 40,000 and an ability to release vasoactive peptides from kininogen in vitro, although the kininogenase activity of different kallikreins is highly variable. The true physiologic role of specific kallikreins is often unrelated to the kininogenase activity. In the mouse a major site of kallikrein synthesis is the male submaxillary gland. Glandular kallikreins are also synthesized in the pancreas and kidney. The several kallikreins found in this tissue include epidermal growth factor-binding protein (EGF-BP) and the gamma subunit of nerve growth factor (NGFG;), which are responsible for the processing of EGF and NGF , respectively.
Gene ID 3817
Alternative KLK2; KLK2A2; MGC12201; hK2; glandular kallikrein 2; kallikrein 2; prostatic
Accession P20151

Image & description

Fig.1. Human Kallikrein-2 (KLK2) Standard Curve.

Fig.1. Human Kallikrein-2 (KLK2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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