Login Register
English
0

Cart

$ 0

Human ADAMTS-like protein 1 (ADAMTSL1) ELISA Kit

Human ADAMTS-like protein 1 (ADAMTSL1) ELISA Kit

Views(63) Publications(0) Catalog no(KTE60925)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Human ADAMTS-like protein 1 (ADAMTSL1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human ADAMTS-like protein 1 (ADAMTSL1) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAMTSL1 in samples. An antibody specific for ADAMTSL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAMTSL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTSL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTSL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ADAMTSL1; RP11-220B22.2; ADAMTSR1; C9orf94; DKFZp686L03130; FLJ35283; FLJ41032; FLJ46891; MGC118803; MGC118805; MGC40193; PUNCTIN; ADAM-TS related protein 1

Product Properties

Kit components • Human ADAMTS-like protein 1 microplate
• Human ADAMTS-like protein 1 standard
• Human ADAMTS-like protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human ADAMTS-like protein 1 (ADAMTSL1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADAMTSL1. No significant cross-reactivity or interference between Human ADAMTSL1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ADAMTSL1 encodes a secreted protein resembling members of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) family. This protein lacks the propeptide region and the metalloproteinase and disintegrin-like domains, which are typical of the ADAMTS family, but contains other ADAMTS domains, including the thrombospondin type 1 motif. This protein may have important functions in the extracellular matrix. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.The deduced 525-amino acid protein contains an N-terminal signal peptide. The mature secreted form contains 497 amino acids and has a calculated molecular mass of about 55 kD.
Gene ID 92949
Alternative ADAMTSL1; RP11-220B22.2; ADAMTSR1; C9orf94; DKFZp686L03130; FLJ35283; FLJ41032; FLJ46891; MGC118803; MGC118805; MGC40193; PUNCTIN; ADAM-TS related protein 1
Accession Q8N6G6

Image & description

Fig.1. Human ADAMTS-like protein 1 (ADAMTSL1) Standard Curve.

Fig.1. Human ADAMTS-like protein 1 (ADAMTSL1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Human ADAMTS-like protein 1 (ADAMTSL1) ELISA Kit”