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Rat Urotensin-2 (UTS2) ELISA Kit

Rat Urotensin-2 (UTS2) ELISA Kit

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Specification

Product name Rat Urotensin-2 (UTS2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Urotensin-2 (UTS2) ELISA Kit employs a two-site sandwich ELISA to quantitate UTS2 in samples. An antibody specific for UTS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUTS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UTS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UTS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative UTS2; PRO1068; U-II; UCN2; UII;

Product Properties

Kit components • Rat Urotensin-2 microplate
• Rat Urotensin-2 standard
• Rat Urotensin-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Urotensin-2 (UTS2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat UTS2. No significant cross-reactivity or interference between Rat UTS2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background A novel peptide, UII-related peptide (URP), from the extract of the rat brain as the sole immunoreactive substance to anti-UII antibody. cDNAs encoding rat, mouse, and human precursor proteins for URP were cloned and revealed that the sequences of mouse and human URP peptides are the same as that for rat URP. Prepro-URP gene is expressed in several rat tissues such as those of the thymus, spleen, testis, and spinal cord, although with lower levels than the prepro-UII gene. In the human, the prepro-URP gene is expressed comparably to prepro-UII in several tissues except the spinal cord. URP was found to bind and activate the human or rat UII receptors (GPR14) and showed a hypotensive effect when administered to anesthetized rats. URP is the endogenous and functional ligand for UII receptor in the rat and mouse, and possibly in the human.
Gene ID 29180
Alternative UTS2; PRO1068; U-II; UCN2; UII;
Accession Q9QZQ4

Image & description

Fig.1. Rat Urotensin-2 (UTS2) Standard Curve.

Fig.1. Rat Urotensin-2 (UTS2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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