|Product name||Human Angio-associated migratory cell protein (AAMP) ELISA Kit|
|Applications notes||This Human Angio-associated migratory cell protein (AAMP) ELISA Kit employs a two-site sandwich ELISA to quantitate AAMP in samples. An antibody specific for AAMP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAAMP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AAMP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AAMP bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Human Angio-associated migratory cell protein microplate
• Human Angio-associated migratory cell protein standard
• Human Angio-associated migratory cell protein detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Human Angio-associated migratory cell protein (AAMP) ELISA Kit has high sensitivity and excellent specificity for detection of Human AAMP. No significant cross-reactivity or interference between Human AAMP and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||AAMP is an immunoglobulin-type protein. It is found to be expressed strongly in endothelial cells, cytotrophoblasts, and poorly differentiated colon adenocarcinoma cells found in lymphatics. The protein contains a WD40 domain and a heparin-binding domain which mediates heparin-sensitive cell adhesion.The predicted 452-amino acid, 49-kD protein contains a heparin-binding consensus sequence near the N terminus, an acidic region, immunoglobulin-like domains which identify it as an immunoglobulin superfamily member, 6 repeats with varying degrees of similarity to the WD40 repeat, and 1 potential transmembrane region. The authors detected a 1.6-kb AAMP transcript and a 52-kD AAMP protein in human tissues and cells.|
Fig.1. Human Angio-associated migratory cell protein (AAMP) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.