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Factor VIII Polyclonal Antibody

Factor VIII Polyclonal Antibody

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Specification

Product name Factor VIII Polyclonal Antibody
Immunogen Synthesized peptide derived from the C-terminal region of human Factor VIII at AA range: 2130-2210
Host Rabbit
Reactivity Human, Mouse
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative F8; F8C; Coagulation factor VIII; Antihemophilic factor; AHF; Procoagulant component

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background F8 encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. F8 produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. DefectsF8 results in hemophilia A, a common recessive X-linked coagulation disorder.
Gene ID 2157
Alternative F8; F8C; Coagulation factor VIII; Antihemophilic factor; AHF; Procoagulant component
Others Factor VIII Polyclonal Antibody detects endogenous levels of Factor VIII protein.
Accession P00451
Observed Band(KD) 300

Image & description

Fig.1. Western Blot analysis of various cells using Factor VIII Polyclonal Antibody.

Fig.1. Western Blot analysis of various cells using Factor VIII Polyclonal Antibody.

Fig.2. Western Blot analysis of HuvEc cells using Factor VIII Polyclonal Antibody.

Fig.2. Western Blot analysis of HuvEc cells using Factor VIII Polyclonal Antibody.

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