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ErbB-3 Polyclonal Antibody

ErbB-3 Polyclonal Antibody

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Specification

Product name ErbB-3 Polyclonal Antibody
Immunogen Synthesized peptide derived from the N-terminal region of human ErbB-3 at AA rangle: 1-80
Host Rabbit
Reactivity Human
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative ERBB3; HER3; Receptor tyrosine-protein kinase erbB-3; Proto-oncogene-like protein c-ErbB-3; Tyrosine kinase-type cell surface receptor HER3

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ERBB3 encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.
Gene ID 2065
Alternative ERBB3; HER3; Receptor tyrosine-protein kinase erbB-3; Proto-oncogene-like protein c-ErbB-3; Tyrosine kinase-type cell surface receptor HER3
Others ErbB-3 Polyclonal Antibody detects endogenous levels of ErbB-3 protein.
Accession P21860
Observed Band(KD) 148

Image & description

Fig.1. Western Blot analysis of 293T cells using ErbB-3 Polyclonal Antibody diluted at 1:2000.

Fig.1. Western Blot analysis of 293T cells using ErbB-3 Polyclonal Antibody diluted at 1:2000.

Fig.2. Western Blot analysis of k562 Mouse-heart Mouse-spleen Mouse-lung cells using ErbB-3 Polyclonal Antibody diluted at 1:2000.

Fig.2. Western Blot analysis of k562 Mouse-heart Mouse-spleen Mouse-lung cells using ErbB-3 Polyclonal Antibody diluted at 1:2000.

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