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Rat Thyrotropin-releasing hormone (TRH) ELISA Kit

Rat Thyrotropin-releasing hormone (TRH) ELISA Kit

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Product name Rat Thyrotropin-releasing hormone (TRH) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Thyrotropin-releasing hormone (TRH) ELISA Kit employs a two-site sandwich ELISA to quantitate TRH in samples. An antibody specific for TRH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TRH; MGC125964; MGC125965;

Product Properties

Kit components • Rat Thyrotropin-releasing hormone microplate
• Rat Thyrotropin-releasing hormone standard
• Rat Thyrotropin-releasing hormone detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Thyrotropin-releasing hormone (TRH) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TRH. No significant cross-reactivity or interference between Rat TRH and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Thyrotropin-releasing hormone (TRH) acts as a stimulator of hormone secretion from adenohypophyseal cells, and its signals are inactivated by TRH-degrading ectoenzyme. The deduced 1,024-amino acid type II integral transmembrane protein is 96% identical to the rat protein. It has 12 putative C-terminal N-glycosylation sites, a potential tyrosine sulfation site (residue 380), a consensus sequence for zinc-dependent metallopeptidases, and a short N-terminal intracellular domain with a potential phosphorylation site. Northern blot analysis revealed expression of 7.5-, 7.0-, 6.0-, and 4.5-kb transcripts. Strongest expression was in brain, with weaker expression in heart, lung, liver, and skeletal muscle, and no expression in kidney and placenta.
Gene ID 25569
Alternative TRH; MGC125964; MGC125965;
Accession P01150

Image & description

Fig.1. Rat Thyrotropin-releasing hormone (TRH) Standard Curve.

Fig.1. Rat Thyrotropin-releasing hormone (TRH) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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