| Product name | Human MAD2L1-binding protein (MAD2L1BP) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human MAD2L1-binding protein (MAD2L1BP) ELISA Kit employs a two-site sandwich ELISA to quantitate MAD2L1BP in samples. An antibody specific for MAD2L1BP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAD2L1BP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAD2L1BP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAD2L1BP bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | MAD2L1BP; CMT2; KIAA0110; MGC11282; RP1-261G23.6; caught by MAD2 protein |
| Kit components | • Human MAD2L1-binding protein microplate • Human MAD2L1-binding protein standard • Human MAD2L1-binding protein detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human MAD2L1-binding protein (MAD2L1BP) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAD2L1BP. No significant cross-reactivity or interference between Human MAD2L1BP and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | MAD2L1BPwas identified as a binding protein of the MAD2 mitotic arrest deficient-like 1 (MAD2/MAD2L1). This protein may interact with the spindle checkpoint and coordinate cell cycle events in late mitosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. Upon the completion of spindle attachment, formation of the CMT2-MAD2 complex coincides with dissociation of the p55CDC-MAD2 complex. Overexpression of CMT2 in cells arrested by the spindle checkpoint causes premature destruction of securin and allows exit from mitosis without chromosome segregation. Depletion of CMT2 induces cell death following a transient delay in the onset of anaphase. These results indicate that CMT3 interacts with the spindle checkpoint and coordinates cell cycle events in late mitosis. |
| Gene ID | 9587 |
| Alternative | MAD2L1BP; CMT2; KIAA0110; MGC11282; RP1-261G23.6; caught by MAD2 protein |
| Accession | Q15013 |
Fig.1. Human MAD2L1-binding protein (MAD2L1BP) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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