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Dynamin I (phospho Ser774) Polyclonal Antibody

Dynamin I (phospho Ser774) Polyclonal Antibody

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Product name Dynamin I (phospho Ser774) Polyclonal Antibody
Immunogen Synthesized peptide derived from human Dynamin I around the phosphorylation site of S774
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative DNM1; DNM; Dynamin-1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background DNM1 encodes a member of the dynamin subfamily of GTP-binding proteins. Dynamin-1 possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described.
Gene ID 1759
Alternative DNM1; DNM; Dynamin-1
Others Phospho-Dynamin I (S774) Polyclonal Antibody detects endogenous levels of Dynamin I protein only when phosphorylated at S774.
Accession Q05193
Observed Band(KD) 97

Image & description

Fig. Western Blot analysis of Mouse cells using Phospho-Dynamin I (S774) Polyclonal Antibody.

Fig. Western Blot analysis of Mouse cells using Phospho-Dynamin I (S774) Polyclonal Antibody.

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