Login Register
English
0

Cart

$ 0

Anti-V5 Tag Mouse Monoclonal Antibody (11D5)

Anti-V5 Tag Mouse Monoclonal Antibody (11D5)

Views(761) Publications(0) Catalog no(ABT2170)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Anti-V5 Tag Mouse Monoclonal Antibody (11D5)
Immunogen Synthetic Peptide
Host Mouse
Reactivity Mammals, Bacteria
Applications IF, IP, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000), IF (1:1000), IP (1:200).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative GKPIPNPLLGLDST epitope; GKPIPNPLLGLDST tag; Protein Rev; Regulator of expression of viral proteins; rev; V5 epitope

Product Properties

Formulation Liquid solution
Storage buffer Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag antibody can be helpful in detecting the recombinant proteins, some of which include transmembrane and secreted proteins fusion protein. This V5-tag antibody is prepared by immunizing synthetic peptide GKPIPNPLLGLDST coupled to KLH.
Alternative GKPIPNPLLGLDST epitope; GKPIPNPLLGLDST tag; Protein Rev; Regulator of expression of viral proteins; rev; V5 epitope

Image & description

Fig.1. Immunofluorescence staining (1:1000) of V5 tag fusion protein in 293 cells with red and counterstained with DAPI.

Fig.1. Immunofluorescence staining (1:1000) of V5 tag fusion protein in 293 cells with red and counterstained with DAPI.

Fig.2. Western blot analysis of 1ug V5 fusion protein with Anti-V5 mouse monoclonal antibody in 1:5000 (lane A) and 1:10000 (lane B) dilutions.

Fig.2. Western blot analysis of 1ug V5 fusion protein with Anti-V5 mouse monoclonal antibody in 1:5000 (lane A) and 1:10000 (lane B) dilutions.

Fig.3. IP (1:200)-WB (1:5000) analysis of V5-Tag fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with V5-tag protein(lane B); IP untransfected 293 cell lysate with Anti V5 tag mAb (lane C); IP transfected 293 cell lysate with normal Mouse IgG (lane D) or with Anti V5 tag mAb (lane E).

Fig.3. IP (1:200)-WB (1:5000) analysis of V5-Tag fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with V5-tag protein(lane B); IP untransfected 293 cell lysate with Anti V5 tag mAb (lane C); IP transfected 293 cell lysate with normal Mouse IgG (lane D) or with Anti V5 tag mAb (lane E).

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Anti-V5 Tag Mouse Monoclonal Antibody (11D5)”