Product name | Anti-V5 Tag Mouse Monoclonal Antibody (11D5) |
Immunogen | Synthetic Peptide |
Host | Mouse |
Reactivity | Mammals, Bacteria |
Applications | IF, IP, WB |
Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000), IF (1:1000), IP (1:200). |
Clonality | Monoclonal |
Preparation method | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
Alternative | GKPIPNPLLGLDST epitope; GKPIPNPLLGLDST tag; Protein Rev; Regulator of expression of viral proteins; rev; V5 epitope |
Formulation | Liquid solution |
Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol. |
Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
Shipping | Gel pack with blue ice. |
Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background | The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag antibody can be helpful in detecting the recombinant proteins, some of which include transmembrane and secreted proteins fusion protein. This V5-tag antibody is prepared by immunizing synthetic peptide GKPIPNPLLGLDST coupled to KLH. |
Alternative | GKPIPNPLLGLDST epitope; GKPIPNPLLGLDST tag; Protein Rev; Regulator of expression of viral proteins; rev; V5 epitope |
Fig.1. Immunofluorescence staining (1:1000) of V5 tag fusion protein in 293 cells with red and counterstained with DAPI.
Fig.2. Western blot analysis of 1ug V5 fusion protein with Anti-V5 mouse monoclonal antibody in 1:5000 (lane A) and 1:10000 (lane B) dilutions.
Fig.3. IP (1:200)-WB (1:5000) analysis of V5-Tag fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with V5-tag protein(lane B); IP untransfected 293 cell lysate with Anti V5 tag mAb (lane C); IP transfected 293 cell lysate with normal Mouse IgG (lane D) or with Anti V5 tag mAb (lane E).
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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