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Human Ryanodine receptor 1 (RYR1) ELISA Kit

Human Ryanodine receptor 1 (RYR1) ELISA Kit

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Specification

Product name Human Ryanodine receptor 1 (RYR1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Ryanodine receptor 1 (RYR1) ELISA Kit employs a two-site sandwich ELISA to quantitate RYR1 in samples. An antibody specific for RYR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRYR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RYR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RYR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative RYR1; CCO; MHS; MHS1; RYDR; RYR; SKRR; central core disease of muscle; ryanodine receptor type1; sarcoplasmic reticulum calcium release channel; skeletal muscle ryanodine receptor; type 1-like ryanodine recep

Product Properties

Kit components • Human Ryanodine receptor 1 microplate
• Human Ryanodine receptor 1 standard
• Human Ryanodine receptor 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Ryanodine receptor 1 (RYR1) ELISA Kit has high sensitivity and excellent specificity for detection of Human RYR1. No significant cross-reactivity or interference between Human RYR1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Ryanodine receptor 1 is a ryanodine receptor found in skeletal muscle. The encoded protein functions as a calcium release channel in the sarcoplasmic reticulum but also serves to connect the sarcoplasmic reticulum and transverse tubule. Mutations in this gene are associated with malignant hyperthermia susceptibility, central core disease, and minicore myopathy with external ophthalmoplegia. Alternatively spliced transcripts encoding different isoforms have been described. Communication between transverse-tubules and sarcoplasmic reticulum. Contraction of skeletal muscle is triggered by release of calcium ions from SR following depolarization of T-tubules.Skeletal muscle and brain (cerebellum and hippocampus).
Gene ID 6261
Alternative RYR1; CCO; MHS; MHS1; RYDR; RYR; SKRR; central core disease of muscle; ryanodine receptor type1; sarcoplasmic reticulum calcium release channel; skeletal muscle ryanodine receptor; type 1-like ryanodine recep
Accession P21817

Image & description

Fig.1. Human Ryanodine receptor 1 (RYR1) Standard Curve.

Fig.1. Human Ryanodine receptor 1 (RYR1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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