MYPT1 (phospho Thr853) Polyclonal Antibody (ABP55324): Site-Specific Precision for RhoA/ROCK Signaling Research
The MYPT1 Phosphorylation Puzzle
MYPT1 (Myosin phosphatase target subunit 1), encoded by PPP1R12A, is the regulatory subunit of myosin phosphatase—the enzyme that dephosphorylates myosin light chain (MLC) and drives smooth muscle relaxation and non-muscle cell relaxation[reference:5]. The RhoA/ROCK pathway phosphorylates MYPT1 at Thr853, inactivating the phosphatase and tilting the balance toward contraction[reference:6].
This Thr853 phosphorylation event is the molecular readout of RhoA/ROCK activity—a pathway implicated in hypertension, cancer cell invasion, and fibrosis. Yet site-specific antibodies for Thr853 face a fundamental challenge: cross-reactivity with total MYPT1 or other phospho-sites (Thr696, Ser852) that can distort the apparent activation state of the pathway.
Site-Specific Immunogen Design
Abbkine's MYPT1 (phospho Thr853) Polyclonal Antibody (ABP55324) is raised against a synthesized peptide derived from human MYPT1 around the phosphorylation site of T853[reference:7]. The antibody is affinity-purified from rabbit antiserum using the epitope-specific immunogen[reference:8], ensuring enrichment for clones that discriminate the Thr853-phosphorylated form.
Validated Applications and Performance
| Application | Suggested Dilution |
|---|---|
| Western Blot | 1:500–1:2000 |
| Immunofluorescence | 1:50–1:200 |
| IHC | 1:100–1:300 |
| ELISA | 1:5000 |
Specifications at a Glance
| Parameter | ABP55324 |
|---|---|
| Target | MYPT1 phosphorylated at Thr853 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Clonality | Polyclonal |
| Concentration | 1 mg/mL |
| Molecular Weight | 130 kDa |
| Formulation | PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide |
| Stability | 1 year at -20°C |
Head-to-Head: Phospho-MYPT1 Antibody Comparison
| Feature | ABP55324 | Generic Phospho-Antibodies |
|---|---|---|
| Epitope Specificity | Thr853 site-specific | Variable; often cross-react with total MYPT1 |
| Affinity Purification | Epitope-specific | Variable |
| Applications | IF, WB, IHC, ELISA | Often limited to WB |
| Species Reactivity | Human, Mouse, Rat | Often restricted |
The Bottom Line
For researchers mapping RhoA/ROCK-driven contractility in vascular smooth muscle, cancer invasion, or fibrotic disease, ABP55324 delivers the site-specific precision required to quantify MYPT1 Thr853 phosphorylation as a clean, unambiguous readout of pathway activation.
📎 Reference: MYPT1 (phospho Thr853) Polyclonal Antibody (ABP55324)
