DRP1 Polyclonal Antibody (ABP51203): Precision Epitope Targeting for Mitochondrial Fission Research
The DRP1 Phospho-Site Cross-Reactivity Trap
DRP1 (Dynamin-related protein 1), encoded by DNM1L, is the master GTPase governing mitochondrial and peroxisomal fission—a process intimately linked to apoptosis, programmed necrosis, and the pathogenesis of neurological disorders including Alzheimer's disease and lethal encephalopathy due to defective mitochondrial fission[reference:0]. Quantifying DRP1 accurately is therefore foundational to mitochondrial dynamics research.
Yet a persistent problem plagues conventional DRP1 antibodies: cross-reactivity with the phosphorylated form of DRP1. The S637 phosphorylation site on DRP1 is a critical regulatory switch—phosphorylation at S637 inhibits fission, while dephosphorylation activates it. Antibodies raised against full-length DRP1 or phospho-peptides often fail to distinguish total DRP1 from its inactive, S637-phosphorylated pool, generating signal that conflates two functionally opposing states.
Epitope-Exclusive Design: Non-Phosphorylation Site of S637
Abbkine's DRP1 Polyclonal Antibody (ABP51203) sidesteps this trap through strategic immunogen design. The antibody is raised against a synthesized peptide derived from human DRP1 around the non-phosphorylation site of S637[reference:1]. This epitope selection ensures that the antibody recognizes total DRP1 irrespective of its S637 phosphorylation status—making it the ideal reagent for measuring DRP1 protein levels without the confounding influence of the phosphorylation switch.
Validated Performance Across Four Applications
ABP51203 is affinity-purified from rabbit antiserum by affinity-chromatography using the epitope-specific immunogen[reference:2], yielding a reagent validated for IF, WB, IHC, and ELISA:
| Application | Suggested Dilution | Validation Data |
|---|---|---|
| Western Blot | 1:500–1:2000 | Detects endogenous DRP1 at ~80 kDa in HeLa and HepG2 lysates (1:500)[reference:3] |
| Immunofluorescence | 1:50–1:200 | Mitochondrial localization confirmed in mouse kidney tissue (1:200, 4°C overnight)[reference:4] |
| IHC | 1:100–1:300 | Validated in tissue sections |
| ELISA | 1:10,000 | High-titer performance |
Broad Species Reactivity and Rigorous Quality Control
| Parameter | ABP51203 |
|---|---|
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Clonality | Polyclonal |
| Concentration | 1 mg/mL |
| Molecular Weight | 80 kDa |
| Formulation | PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide |
| Stability | 1 year at -20°C |
Head-to-Head: DRP1 Antibody Comparison
| Feature | ABP51203 | Conventional DRP1 Antibodies |
|---|---|---|
| Epitope | Non-phosphorylation site of S637 | Often full-length or phospho-peptides |
| Phospho-S637 Interference | Minimized by design | Frequently present |
| Applications | IF, WB, IHC, ELISA | Variable |
| Species Reactivity | Human, Mouse, Rat | Often limited |
| Affinity Purification | Yes (epitope-specific) | Variable |
The Bottom Line
For researchers dissecting mitochondrial fission dynamics—from neurodegenerative disease models to cancer metabolism—ABP51203 delivers the epitope precision needed to distinguish total DRP1 from its regulatory phospho-isoforms, ensuring your mitochondrial dynamics data reflects biology, not antibody artifact.
📎 Reference: DRP1 Polyclonal Antibody (ABP51203)
