CD4 Monoclonal Antibody (ABM40070): Monoclonal Consistency for T-Cell and Immune Microenvironment Studies
The CD4 Antibody Variability Problem
CD4 is the canonical marker of helper T cells—a membrane glycoprotein that interacts with MHC class II antigens, serves as the receptor for HIV, and functions to initiate or augment early T-cell activation[reference:9]. CD4 is also expressed beyond T lymphocytes, in B cells, macrophages, granulocytes, and specific brain regions, making it a versatile but demanding immunohistochemical target[reference:10].
The challenge for CD4 immunohistochemistry is reproducibility. Polyclonal anti-CD4 antibodies, by nature, comprise a heterogeneous pool of immunoglobulins with varying affinities for different epitopes, leading to lot-to-lot variability in staining intensity and cellular specificity. For longitudinal studies or multi-center collaborations, this variability is a silent source of irreproducible data.
Monoclonal Advantage: Single-Epitope Consistency
Abbkine's CD4 Monoclonal Antibody (ABM40070) is a mouse monoclonal antibody, affinity-purified from mouse ascites by affinity-chromatography using a specific immunogen[reference:11]. Because every antibody molecule in the vial recognizes the same epitope with identical affinity, the reagent eliminates the epitope-distribution drift that polyclonal reagents inevitably exhibit.
Validated Immunohistochemistry Across Three Species
ABM40070 is validated for IHC with a suggested starting dilution of 1:200[reference:12]. The product page documents IHC validation across:
| Tissue | Species | Protocol |
|---|---|---|
| Stomach | Human | Citrate pH 6.0 retrieval, >98°C 20 min; primary 1:200, 4°C overnight[reference:13] |
| Brain | Mouse | Same retrieval and dilution[reference:14] |
| Kidney | Rat | Same retrieval and dilution[reference:15] |
This cross-species validation (human, mouse, rat) is a critical advantage for translational research spanning model organisms and clinical specimens.
Product Specifications
| Parameter | ABM40070 |
|---|---|
| Target | CD4 |
| Host | Mouse |
| Clonality | Monoclonal |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC (1:200) |
| Concentration | 1 mg/mL |
| Formulation | PBS pH 7.4, 0.5% BSA, 0.02% sodium azide, 50% glycerol |
| Stability | 1 year at -20°C |
Head-to-Head: CD4 Antibody Comparison
| Feature | ABM40070 | Polyclonal Anti-CD4 |
|---|---|---|
| Clonality | Monoclonal | Polyclonal |
| Lot-to-Lot Consistency | High | Variable |
| Cross-species Validation | Human, Mouse, Rat (IHC) | Often limited |
| IHC Documentation | Tissue-specific protocols provided | Variable |
| Epitope Uniformity | Single epitope | Heterogeneous pool |
The Bottom Line
For researchers profiling CD4+ T-cell infiltration in tumor microenvironments, characterizing neuroinflammation, or standardizing immune phenotyping across species, ABM40070 delivers the monoclonal consistency that turns CD4 immunohistochemistry from a variable into a controlled parameter.
📎 Reference: CD4 Monoclonal Antibody (ABM40070)
