Mouse Vitamin A (VA) ELISA Kit — Application Scenarios for Nutrition, Immunology, and Developmental Research
Vitamin A: The Fat-Soluble Signal That Shapes Immunity, Vision, and Organogenesis
Vitamin A is not one molecule — it's a family of fat-soluble retinoids including retinol, retinal, and retinyl esters[reference:33]. Each form has a distinct job: retinal is the chromophore of rhodopsin, the light-absorbing protein in retinal photoreceptors; retinoic acid is a morphogen that patterns the developing heart, lungs, kidneys, and other organs; and retinol circulates as the body's storage and transport form, mobilized from the liver as needed[reference:34].
In mouse models, vitamin A status is a systemic variable that researchers must either control or measure — because vitamin A deficiency (VAD) and excess (hypervitaminosis A) produce profoundly different phenotypes in immune function, organogenesis, vision, and metabolic health[reference:35]. Vitamin A supports cell growth and differentiation, playing a critical role in the normal formation and maintenance of the heart, lungs, kidneys, and other organs[reference:36].
Abbkine's Mouse Vitamin A (VA) ELISA Kit (KTE70032) provides a quantitative tool for measuring vitamin A status in mouse serum, plasma, and other biological samples. Here's how researchers across different fields are putting it to work.
Nutritional Immunology: Vitamin A as a Immune-Modulating Nutrient
Vitamin A is essential for immune function, particularly for maintaining the integrity of mucosal barriers and supporting T cell and B cell responses[reference:37]. Nutritional immunologists use this kit to:
- Quantify vitamin A in serum and plasma from dietary intervention studies
- Correlate vitamin A status with immune cell populations and cytokine profiles
- Study vitamin A deficiency effects on infection susceptibility
- Monitor vitamin A supplementation efficacy in disease models
The kit is validated for serum, plasma, cell culture supernatants, and other biological fluids[reference:38], with a sandwich ELISA format that delivers quantitative results.
Developmental Biology: Retinoic Acid Signaling and Organogenesis
Vitamin A's active metabolite, retinoic acid, is a morphogen that patterns the developing embryo — specifying the anterior-posterior axis, directing heart chamber formation, and guiding lung branching morphogenesis[reference:39]. Developmental biologists use this kit to:
- Quantify vitamin A in embryonic tissue lysates and maternal serum
- Study vitamin A deficiency effects on organogenesis
- Investigate retinoic acid gradient formation and target gene activation
- Monitor vitamin A status in gene knockout models (e.g., RBP4, STRA6)
Metabolic and Liver Research: Vitamin A Storage and Mobilization
The liver is the body's primary vitamin A storage organ, and hepatic stellate cells are the major storage site. Metabolic and hepatology researchers use this kit to:
- Quantify vitamin A in liver tissue lysates and serum
- Study vitamin A mobilization during fasting, inflammation, and liver injury
- Investigate vitamin A metabolism in NAFLD and fibrosis models
- Monitor vitamin A status in obesity and diabetes models
Vision Research: Rhodopsin and Retinal Function
Vitamin A is critical for vision as an essential component of rhodopsin[reference:40]. Vision researchers use this kit to:
- Quantify vitamin A in retinal tissue and serum from mouse models
- Study vitamin A deficiency effects on visual function
- Investigate retinoid cycling in the visual cycle
- Monitor vitamin A status in retinal degeneration models
Why Choose This Kit for Your Vitamin A Research?
| Feature | Benefit |
|---|---|
| Two-site sandwich ELISA | Quantitative, specific detection |
| Mouse reactivity | Directly applicable to mouse models |
| Multiple sample types | Serum, plasma, cell culture supernatants, other biological fluids |
| Biotin-streptavidin HRP amplification | Enhanced signal for reliable detection |
| No significant cross-reactivity | Specific for mouse VA — no interference from analogues[reference:41] |
| Complete kit | Microplate, standards, detection antibody, Streptavidin-HRP, all buffers included[reference:42] |
Kit Components at a Glance
- Mouse Vitamin A microplate (pre-coated with capture antibody)
- Mouse Vitamin A standard
- Mouse Vitamin A detection antibody (biotin-conjugated)
- Streptavidin-HRP
- Standard diluent, assay buffer, HRP substrate, stop solution, wash buffer, plate covers
🔬 Which research field does your work belong to?
Nutritional Immunology | Developmental Biology | Metabolic & Liver Research | Vision Research | Other
Share your application with us — we'd love to hear how you're using this kit in your studies!
📌 Reference Link: Mouse Vitamin A (VA) ELISA Kit — KTE70032
