Human Tyrosine-Protein Kinase Lyn (LYN) ELISA Kit — Technical Comparison & Buying Guide for Immune Signaling and Cancer Research
LYN: The Src-Family Kinase That Decides Whether B Cells Fire or Go Silent
Lyn kinase (LYN, Lck/Yes-related novel tyrosine kinase) is a member of the Src family of cytoplasmic (non-receptor) protein tyrosine kinases that catalyzes the transfer of the gamma-phosphoryl group from ATP to tyrosine residues in protein substrates[reference:40]. Lyn was first identified in 1987 by Yamanashi et al., and its principal function is the transduction of signals after activation of different cellular receptors[reference:41]. Lyn, as well as Fyn and Blk, promotes B cell activation by phosphorylating ITAMs (immunoreceptor tyrosine activation motifs) in CD19 and in Ig components of the BCR — a critical role in adaptive immunity[reference:42].
Yet despite its central role in immune signaling, quantifying Lyn in disease models has been a persistent bottleneck. The Human Tyrosine-Protein Kinase Lyn (LYN) ELISA Kit (KTE61764) from Abbkine is built to give you a quantitative, two-site sandwich ELISA readout of this pivotal kinase.
Why Traditional LYN Detection Methods Fall Short
| Method | Limitation |
|---|---|
| Western Blot | Semi-quantitative; large sample volumes; struggles with low-abundance LYN; 60 kDa band transfer efficiency varies |
| qPCR | Measures mRNA, not protein — fails to capture post-translational regulation and kinase activation |
| Generic ELISA | High structural homology across Src family members (Src, Fyn, Yes, Fgr, Lck) causes cross-reactivity |
The Src kinase family is full of structural lookalikes, and most off-the-shelf ELISA kits target conserved domains, meaning they cross-react with Src, Fyn, or other family members.
Abbkine KTE61764: Engineered for LYN-Specific Precision
The kit uses a two-site sandwich ELISA format with a pre-coated capture antibody and a biotin-conjugated detection antibody, both rigorously validated for exclusive binding to human LYN. The Streptavidin-HRP amplification system boosts sensitivity for low-abundance antigen detection without compromising specificity[reference:43].
Key Specifications
| Parameter | KTE61764 |
|---|---|
| Format | Two-site sandwich ELISA (quantitative) |
| Sample Types | Cell culture supernatants, plasma, serum, other biological fluids |
| Assay Duration | 3–5 hours |
| Specificity | No significant cross-reactivity with LYN analogues[reference:44] |
| Sensitivity | High sensitivity for detection of Human LYN[reference:45] |
Head-to-Head Comparison: KTE61764 vs. Legacy Methods
| Feature | Abbkine KTE61764 | Polyclonal Kits | Western Blot |
|---|---|---|---|
| LYN Specificity | High — no cross-reactivity with Src family members | Low — cross-reacts with Src, Fyn, Yes | Antibody-dependent |
| Quantitative | Yes (standard curve) | Yes (standard curve) | Semi-quantitative |
| Sample Volume | Minimal | 50–100 µL | 20–50 µg protein |
| Throughput | 96-well plate | 96-well plate | Low (gel-based) |
| Reproducibility | High (standardized format) | Variable | Highly variable |
Why Choose Abbkine KTE61764?
- LYN-Specific Detection: Validated antibodies ensure your signal comes from LYN — not Src, Fyn, or other Src family members
- Quantitative Readout: Converts this Src-family kinase into ng/mL with a standard curve
- Biotin-Streptavidin Amplification: Enhanced signal amplification for low-abundance antigen detection
- Broad Sample Compatibility: Works with cell culture supernatants, plasma, serum, and other biological fluids
- Complete Kit: Pre-coated plate, standards, detection antibodies, and all buffers included
- Research-Validated: Purpose-built for immune signaling and cancer research
Who Should Buy This Kit?
✅ Buy KTE61764 if you:
- Study B cell activation, BCR signaling, or ITAM phosphorylation
- Research leukemia, lymphoma, or myeloid malignancies
- Investigate cancer metastasis and treatment resistance (Lyn overexpression contributes to tumor growth and metastasis in leukemia, prostate, breast, lung, and pancreatic cancers)[reference:46]
- Work on G-CSF receptor signaling and myeloid cell biology[reference:47]
- Need to quantify human LYN in serum, plasma, or cell culture supernatants
- Require specificity — no cross-reactivity with other Src family members
- Want a complete, ready-to-use kit for high-throughput screening
❌ Consider alternatives if you:
- Only need qualitative detection (presence/absence)
- Work with non-human samples (mouse, rat) — check Abbkine's other offerings
- Require a faster assay (<3 hours)
📌 Reference Link: Human Tyrosine-Protein Kinase Lyn (LYN) ELISA Kit — KTE61764
