Human LTBP3 ELISA Kit — Application Scenarios for TGF-β Biology, Adipogenesis, and Skeletal Research
LTBP3: The Molecular Librarian of TGF-β Storage
Latent-transforming growth factor beta-binding protein 3 (LTBP3) is a ~130–150 kDa secreted glycoprotein that performs one of the most elegant jobs in extracellular matrix (ECM) biology: it stores TGF-β in a latent, inactive form and releases it on demand when the tissue needs it. LTBP3 covalently binds the Latency-Associated Peptide (LAP), which in turn holds the mature TGF-β1, TGF-β2, or TGF-β3 in a molecular embrace. Together, this ternary complex — LAP–TGF-β–LTBP3 — is anchored to the ECM via LTBP3's interaction with fibrillin microfibrils, keeping a reservoir of the most potent cytokine in the body locked away until proteases, integrins, or mechanical force liberate it.
LTBP3 is not a passive library shelf. It controls TGF-β bioavailability at the tissue level, and its absence or dysfunction shifts the entire TGF-β signaling balance. LTBP3 knockout mice develop an osteopetrosis-like phenotype with impaired skeletal formation and tooth development. LTBP3 is also required for adipogenesis — the differentiation of mesenchymal stem cells into fat cells — through TGF-β-dependent mechanisms. And in Marfan syndrome, disrupted LTBP3–fibrillin interactions contribute to thoracic aortic aneurysm pathogenesis.
Abbkine's Human Latent-transforming growth factor beta-binding protein 3 (LTBP3) ELISA Kit (KTE61770) gives researchers a sensitive, specific tool to quantify this pivotal ECM-embedded cytokine reservoir. Here's how researchers across different fields are putting it to work.
TGF-β Biology and Extracellular Matrix Research
LTBP3 is the molecular switch that determines how much TGF-β reaches the cell surface. ECM biologists use this kit to:
- Quantify LTBP3 in fibroblast-conditioned media and extracellular matrix preparations
- Study LTBP3-mediated TGF-β sequestration and release dynamics
- Investigate the LTBP3–fibrillin interaction in connective tissue assembly
- Monitor LTBP3 levels in fibrotic tissue models
Adipogenesis and Metabolic Research
LTBP3 is required for adipogenesis — the process by which mesenchymal stem cells differentiate into adipocytes. Metabolic researchers use this kit to:
- Quantify LTBP3 during adipocyte differentiation of human MSCs
- Study TGF-β-dependent regulation of adipogenesis
- Investigate LTBP3's role in obesity and metabolic disorders
- Monitor LTBP3 levels in adipose tissue lysates
Skeletal Biology and Developmental Research
LTBP3-deficient mice exhibit an osteopetrosis-like phenotype — thickened bones due to impaired osteoclast function — and defects in tooth development. Skeletal biologists use this kit to:
- Quantify LTBP3 in bone tissue lysates and osteoblast cultures
- Study LTBP3's role in skeletal formation and tooth development
- Investigate LTBP3 in osteoporosis and bone remodeling research
- Monitor LTBP3 during mesenchymal stem cell osteogenic differentiation
Marfan Syndrome and Vascular Research
In Marfan syndrome, defective LTBP3–fibrillin-1 interactions contribute to perturbed TGF-β signaling and thoracic aortic aneurysm pathogenesis. Cardiovascular researchers use this kit to:
- Quantify LTBP3 in aortic tissue lysates and plasma
- Study TGF-β signaling dysregulation in Marfan syndrome
- Investigate LTBP3 as a biomarker for aortic aneurysm risk
- Monitor LTBP3 in fibrillinopathies and connective tissue disorders
Why Choose This Kit for Your LTBP3 Research?
| Feature | Benefit |
|---|---|
| Two-site sandwich ELISA | Quantitative, specific detection |
| Human reactivity | Directly applicable to human samples |
| Biotin-streptavidin HRP amplification | Enhanced signal for low-abundance LTBP3 |
| Multiple sample types | Serum, plasma, cell culture supernatants, biological fluids |
| High specificity | No significant cross-reactivity with LTBP1, LTBP2, or LTBP4 |
| Complete kit | Pre-coated plate, standards, detection antibodies, all buffers included |
Kit Components at a Glance
- Human LTBP3 microplate (pre-coated with capture antibody)
- Human LTBP3 standard
- Human LTBP3 detection antibody (biotin-conjugated)
- Streptavidin-HRP
- Standard diluent, assay buffer, HRP substrate, stop solution, wash buffer, plate covers
🔬 Which research field does your work belong to?
TGF-β Biology | Adipogenesis | Skeletal Biology | Marfan Syndrome & Vascular Research | Other
Share your application with us — we'd love to hear how you're using this kit in your studies!
📌 Reference Link: Human Latent-transforming growth factor beta-binding protein 3 (LTBP3) ELISA Kit — KTE61770
