Human TGFB1I1 ELISA Kit — FAQ for Cell Signaling, Fibrosis, and Cancer Researchers

Human TGFB1I1 ELISA Kit: Your Questions Answered

Transforming growth factor beta-1-induced transcript 1 protein (TGFB1I1) , also known as TSC-22 or TGFB1I1, is a protein that is induced by TGF-β1 signaling. TGFB1I1 is a member of the TSC-22/Dip/Bun family of leucine zipper transcription factors and plays important roles in cell growth regulation, apoptosis, fibrosis, and tumor suppression. It is involved in the regulation of TGF-β signaling pathways and has been implicated in cancer, fibrotic diseases, and inflammatory disorders.

Abbkine's Human Transforming growth factor beta-1-induced transcript 1 protein (TGFB1I1) ELISA Kit (KTE60509) provides researchers with a reliable tool for quantifying this important TGF-β-induced protein in human samples. Here are answers to the most common questions about this kit and TGFB1I1 detection.

What is the detection principle of this TGFB1I1 ELISA kit?

This kit employs a two-site sandwich ELISA to quantitate TGFB1I1 in samples. An antibody specific for TGFB1I1 has been pre-coated onto a microplate. When you add standards and samples, any TGFB1I1 present binds to the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGFB1I1 is added. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added and color develops in proportion to the amount of TGFB1I1 bound. The color development is stopped and the intensity is measured — providing a quantitative, colorimetric readout.

What sample types can I use?

The kit is validated for serum, plasma, cell culture supernatants, and other biological fluids. This flexibility makes it suitable for various experimental systems, from cell culture models to clinical research samples.

What is the sensitivity and detection range?

Abbkine's ELISA kits are engineered for high sensitivity and excellent specificity. For specific sensitivity information tailored to your expected concentration range, contact Abbkine's technical support team.

Does this kit cross-react with other TGF-β-induced proteins or TSC-22 family members?

No significant cross-reactivity or interference between human TGFB1I1 and analogues has been observed. This specificity ensures you're measuring TGFB1I1 specifically — without interference from related proteins.

How should I store and handle the kit?

• Unopened kit: Store at 2–8°C
• Opened kit: Follow protocol-specific storage instructions
• Shipping: Gel pack with blue ice maintains temperature during transit

Critical handling tips:

• Allow all reagents to warm to room temperature for at least 30 minutes before opening
• Pre-rinse pipette tips with reagent; use fresh tips for each sample, standard, and reagent
• Mix thoroughly — use a low-frequency oscillator or gentle hand shaking every 10 minutes
• Run samples and standards in duplicate or triplicate
• Keep unused wells desiccated at 4°C in the provided sealed bag

What applications is this kit suitable for?

This kit is designed for research use only — not for human or clinical diagnosis. Applications include:

• TGF-β signaling pathway research
• Fibrosis and wound healing studies
• Cancer research (tumor suppression, epithelial-mesenchymal transition)
• Cell growth and apoptosis regulation studies
• Inflammatory disease research
• Drug development (TGF-β pathway modulators)

What's included in the kit?

A complete, ready-to-use kit.

📌 Reference Link: Human Transforming growth factor beta-1-induced transcript 1 protein (TGFB1I1) ELISA Kit — KTE60509