Human Malectin (MLEC) ELISA Kit — FAQ for ER Homeostasis and Glycosylation Researchers

Human Malectin (MLEC) ELISA Kit: Your Questions Answered

Malectin (MLEC) is a 32-kDa Type I membrane-anchored carbohydrate-binding protein that resides in the endoplasmic reticulum (ER) of every eukaryotic cell. It binds with high affinity and exquisite specificity to Glc₂Man₉GlcNAc₂ (G2M9) N-glycans — the exact oligosaccharide structure that appears on newly synthesized glycoproteins before they are trimmed by ER glucosidases I and II. Malectin sits at the crossroads of glycoprotein quality control, ER-associated degradation (ERAD) , and ER homeostasis maintenance — functions directly linked to diabetes, neurodegenerative diseases, and cancer where ER stress and glycosylation dysregulation drive disease progression[reference:0].

Abbkine's Human Malectin (MLEC) ELISA Kit (KTE61601) is the tool built for exactly this problem. Here are answers to the most common questions about this kit and MLEC detection.

What is the detection principle of this MLEC ELISA kit?

This kit employs a two-site sandwich ELISA to quantitate MLEC in samples. A highly specific capture antibody is pre-coated onto the microplate. When standards and samples are added, any MLEC present binds to the immobilized antibody. After removing unbound substances, a biotin-conjugated detection antibody specific for MLEC is added. Following washing, Streptavidin-HRP is added, and a chromogen solution produces color in proportion to the amount of MLEC bound. The color development is stopped and the intensity is measured at the appropriate wavelength[reference:1].

What sample types can I use?

The kit is validated for serum, plasma, cell culture supernatants, and other biological fluids — the primary sample types for translational MLEC studies[reference:2].

What is the sensitivity and detection range?

The kit is validated with a standard curve R²=0.9939 — one of the most rigorously validated ELISAs on the market[reference:3]. Abbkine's ELISA kits are engineered for high sensitivity and excellent specificity. For specific sensitivity information, contact Abbkine's technical support team.

Does this kit cross-react with other lectins or carbohydrate-binding proteins?

No significant cross-reactivity or interference between human MLEC and analogues has been observed. The kit uses antibodies that recognize unique epitopes of human MLEC (not shared with homologous proteins), eliminating false signals from other lectins such as galectins — a critical advantage for complex samples like plasma or serum[reference:4].

What are the key features and benefits?

What applications is this kit suitable for?

This kit is designed for research use only. Applications include:

• ER homeostasis and glycoprotein quality control research
• N-glycosylation regulation and protein folding studies
• ER stress and ERAD research
• Diabetes and neurodegenerative disease research (ER stress-related)
• Cancer biology (glycosylation dysregulation)
• Coronavirus replication research (MLEC promotes viral protein biogenesis)[reference:8]

What's included in the kit?

A complete, ready-to-use kit. Priced at $339 for a 48T kit — offering unparalleled value for ER biology researchers[reference:9].

📌 Reference Link: Human Malectin (MLEC) ELISA Kit — KTE61601