Human Lipopolysaccharides (LPS) ELISA Kit — FAQ for Sepsis, Gut Barrier, and Endotoxin Researchers

Human LPS ELISA Kit: Your Questions Answered

Lipopolysaccharides (LPS) , also known as endotoxins, are large glycolipid molecules found in the outer membrane of Gram-negative bacteria. LPS is the canonical pathogen-associated molecular pattern (PAMP) recognized by the innate immune system through TLR4, triggering the inflammatory cascade that defines sepsis, septic shock, and acute respiratory distress syndrome. LPS is also a critical marker of gut barrier permeability — when the intestinal epithelial barrier fails, LPS leaks into the bloodstream, a phenomenon known as metabolic endotoxemia, implicated in obesity, insulin resistance, and chronic inflammation.

Abbkine's Human Lipopolysaccharides (LPS) ELISA Kit (KTE62375) provides researchers with a sensitive and specific tool for quantifying LPS in human samples. Here are answers to the most common questions about this kit and LPS detection.

What is the detection principle of this LPS ELISA kit?

This kit employs a sandwich ELISA to quantitate LPS in samples. An antibody specific for LPS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LPS present is bound by the immobilized antibody. After removing any unbound substances, a detection antibody is added, followed by washing and a substrate solution. Color develops in proportion to the amount of LPS bound, and the intensity is measured at the appropriate wavelength.

What sample types can I use?

The kit is validated for serum, plasma, cell culture supernatants, and other biological fluids. This flexibility makes it suitable for sepsis research, gut permeability studies, and clinical translational investigations.

What is the sensitivity and detection range?

Abbkine's ELISA kits are engineered for high sensitivity and excellent specificity. For specific sensitivity information tailored to your expected concentration range, contact Abbkine's technical support team.

Does this kit cross-react with other bacterial components?

No significant cross-reactivity or interference with other bacterial components has been observed. This specificity ensures you're measuring LPS specifically — critical for distinguishing endotoxin from other PAMPs in complex biological samples.

How should I store and handle the kit?

• Unopened kit: Store at 2–8°C
• Opened kit: Follow protocol-specific storage instructions
• Shipping: Gel pack with blue ice

Critical handling tips:

• Avoid endotoxin contamination — use pyrogen-free labware and reagents at all times
• Process samples promptly and avoid repeated freeze-thaw cycles
• Allow all reagents to warm to room temperature for at least 30 minutes before opening
• Run samples and standards in duplicate or triplicate

What applications is this kit suitable for?

This kit is designed for research use only — not for human or clinical diagnosis. Applications include:

• Sepsis and septic shock research
• Gut barrier permeability and metabolic endotoxemia studies
• Inflammatory bowel disease (IBD) research
• Liver disease (LPS is a key driver of hepatic inflammation)
• Metabolic syndrome and obesity research
• Chronic inflammatory diseases (rheumatoid arthritis, atherosclerosis)
• Vaccine and biologic manufacturing (endotoxin contamination testing)

Why measure LPS?

LPS is a critical molecule at the interface of infection and inflammation:

• It is the primary driver of sepsis — the immune response to LPS causes organ failure
• It is a marker of gut barrier failure — elevated circulating LPS indicates "leaky gut"
• It drives chronic low-grade inflammation in obesity and metabolic disease
• It is a major contaminant in biologic drugs and medical devices

What's included in the kit?

A complete, ready-to-use kit.

📌 Reference Link: Human Lipopolysaccharides (LPS) ELISA Kit — KTE62375