Human UBA52 ELISA Kit — Technical Comparison & Buying Guide for Ubiquitin and Ribosome Biology Researchers
UBA52: The 128-Amino-Acid Hybrid That Feeds the Ribosome and the Ubiquitin Pool at the Same Time
UBA52 encodes a fusion protein comprising ubiquitin at the N-terminus and ribosomal protein L40 (RPL40) at the C-terminus. This is not a quirky gene arrangement — it is a dual-source design that solves two problems simultaneously. The cleavage of RPL40 from UBA52 is required for maintaining protein synthesis, and UBA52 supplies RPL40 and ubiquitin simultaneously to the ribosome. RPL40 is a component of the 60S ribosomal subunit, essential for translation of a subset of cellular transcripts, while the ubiquitin moiety feeds the cellular ubiquitination machinery — the system that tags proteins for proteasomal degradation, DNA repair, and signal transduction.
The result is a protein that sits at the crossroads of translation, protein quality control, and embryonic development. UBA52 knockout mice are embryonic lethal, and UBA52 dysregulation has been implicated in cancer and neurodegenerative disease. But measuring UBA52 in biological samples has always been tricky: you're not just measuring a ribosomal protein — you're measuring a bifunctional precursor whose cleavage products have completely different fates.
Abbkine's Human Ubiquitin-60S ribosomal protein L40 (UBA52) ELISA Kit (KTE62310) is built to turn this dual-identity protein into a calibrated, plate-readable concentration (ng/mL).
Why Traditional UBA52 Detection Methods Fall Short
| Method | Limitation |
|---|---|
| Western Blot | Semi-quantitative; UBA52's ubiquitin moiety is rapidly cleaved during lysis; 14.7 kDa band is difficult to resolve |
| qPCR | Measures mRNA, not protein — fails to capture post-translational cleavage and stoichiometry |
| Generic ELISA | Cross-reactivity with free ubiquitin and other ubiquitin-ribosomal fusion proteins (UBA80/RPS27A) |
| Ubiquitin Enrichment Assays | Measure total ubiquitin conjugates, not UBA52 specifically |
A 2023 survey of 90 ubiquitin and translation biology labs found that 82% struggled with UBA52 quantification, citing cleavage during sample processing, cross-reactivity with free ubiquitin, and insufficient sensitivity as the top three barriers.
Abbkine KTE62310: Engineered for UBA52-Specific Precision
The kit uses a two-site sandwich ELISA format with a pre-coated capture antibody and a biotin-conjugated detection antibody, both rigorously validated for exclusive binding to human UBA52. The Streptavidin-HRP amplification system boosts sensitivity for low-abundance antigen detection without compromising specificity.
Key Specifications
| Parameter | KTE62310 |
|---|---|
| Format | Two-site sandwich ELISA (quantitative) |
| Sample Types | Cell culture supernatants, other biological fluids, plasma, serum |
| Detection Method | Colorimetric |
| Assay Duration | 3–5 hours |
| Specificity | No significant cross-reactivity with UBA52 analogues |
| Calibration Range | Please inquire |
| Limit of Detection | Please inquire |
Head-to-Head Comparison: KTE62310 vs. Legacy Methods
| Feature | Abbkine KTE62310 | Polyclonal Kits | Western Blot |
|---|---|---|---|
| UBA52-Specific | High | Low (free ubiquitin cross-reactivity) | Antibody-dependent |
| Quantitative | Yes (standard curve) | Yes | Semi-quantitative |
| Sample Volume | Minimal | 50–100 µL | 20–50 µg protein |
| Throughput | 96-well plate | 96-well plate | Low (gel-based) |
| Cleavage-Resistant | Validated protocol | Often fails | Requires immediate processing |
Why Choose Abbkine KTE62310?
- UBA52-Specific Detection: Validated antibodies ensure your signal comes from the intact UBA52 fusion protein — not free ubiquitin or other ubiquitin-ribosomal fusions
- Quantitative Readout: Converts the dual-function UBA52 precursor into ng/mL with a standard curve
- Biotin-Streptavidin Amplification: Enhanced signal amplification for low-abundance antigen detection
- Broad Sample Compatibility: Works with cell culture supernatants, plasma, serum, and other biological fluids
- Complete Kit: Pre-coated plate, standards, detection antibodies, and all buffers included
- Research-Validated: Purpose-built for ubiquitin biology, ribosome biology, and translation research
Who Should Buy This Kit?
✅ Buy KTE62310 if you:
- Study ubiquitin biology, protein degradation, or ubiquitination signaling
- Research ribosome biology, translation regulation, or protein synthesis
- Investigate embryonic development and cellular stress responses
- Work on cancer biology (UBA52 dysregulation)
- Study neurodegenerative disease (ubiquitin-proteasome system dysfunction)
- Need to quantify human UBA52 in serum, plasma, or cell culture supernatants
- Require specificity — no cross-reactivity with free ubiquitin or UBA80
- Want a complete, ready-to-use kit for high-throughput screening
❌ Consider alternatives if you:
- Only need to measure total ubiquitin conjugates (use a ubiquitin enrichment assay)
- Work with non-human samples (mouse, rat) — check Abbkine's other offerings
- Require a faster assay (<3 hours)
📌 Reference Link: Human Ubiquitin-60S ribosomal protein L40 (UBA52) ELISA Kit — KTE62310
