Human Collagen Type III Alpha 1 (COL3A1) ELISA Kit — Application Scenarios for Fibrosis and Cancer Research

COL3A1: The 140-kDa Fibrillar Backbone of Every Scar and Every Tumor Stroma

Collagen Type III Alpha 1 (COL3A1) is the primary component of type III collagen — a homotrimeric fibrillar collagen that assembles into the soft, extensible fibrils found in blood vessels, skin, and internal organs. Type III collagen is the "first responder" collagen: during wound healing and tissue repair, fibroblasts deposit type III collagen before type I collagen replaces it in mature scar tissue. This makes COL3A1 a direct molecular readout of active fibrogenesis — the rate at which new collagen is being laid down.

COL3A1 dysregulation is implicated across a spectrum of diseases. In liver fibrosis, COL3A1 is upregulated 5–10 fold. In idiopathic pulmonary fibrosis (IPF) , COL3A1 is a hub gene in the fibrotic network. In cancer, COL3A1 is a component of tumor-associated aligned collagen and a marker of cancer-associated fibroblasts (CAFs) — the stromal cells that remodel the tumor microenvironment and promote invasion. Mutations in COL3A1 cause vascular Ehlers-Danlos syndrome (vEDS) , a life-threatening connective tissue disorder characterized by arterial rupture and organ fragility.

Abbkine's Human Collagen Type III Alpha 1 (COL3A1) ELISA Kit (KTE62513) delivers a calibration range of 37.5–600 pg/mL and a limit of detection of 1.0 pg/mL — sensitivity sufficient to detect active fibrogenesis in serum, plasma, and cell culture supernatants. Here's how researchers across different fields are putting it to work.

Liver Fibrosis Research: Measuring Active Scar Formation

COL3A1 is one of the most highly upregulated genes in hepatic fibrosis. Hepatology researchers use this kit to:

• Quantify COL3A1 in serum and plasma from liver fibrosis patients
• Monitor fibrogenesis dynamics in response to anti-fibrotic therapies
• Study hepatic stellate cell activation and collagen deposition
• Correlate COL3A1 levels with fibrosis staging (Ishak, METAVIR)

The kit's limit of detection of 1.0 pg/mL captures the low-level COL3A1 elevation that marks early fibrogenesis — before clinical symptoms appear.

Cancer Research: Tumor Stroma and CAF Biology

COL3A1 is a marker of cancer-associated fibroblasts (CAFs) and a component of tumor-associated aligned collagen. Oncology researchers use this kit to:

• Quantify COL3A1 in tumor tissue lysates and CAF-conditioned media
• Study tumor fibrosis and stromal remodeling
• Investigate COL3A1 as a prognostic biomarker across solid tumor types
• Monitor CAF-mediated collagen deposition in 3D culture models

Pulmonary and Cardiac Fibrosis Research

COL3A1 is a hub gene in IPF and a key driver of cardiac fibrosis. Pulmonary and cardiovascular researchers use this kit to:

• Quantify COL3A1 in lung tissue lysates and BALF
• Study cardiac fibrosis and adverse remodeling post-MI
• Investigate fibrotic activity as a prognostic biomarker
• Monitor anti-fibrotic treatment response

Vascular Ehlers-Danlos Syndrome (vEDS) Research

Mutations in COL3A1 cause vascular Ehlers-Danlos syndrome, characterized by arterial rupture and organ fragility. Rare disease researchers use this kit to:

• Quantify COL3A1 in fibroblast cultures from vEDS patients
• Study collagen synthesis and secretion defects
• Investigate genotype-phenotype correlations in COL3A1 mutations

Why Choose This Kit for Your COL3A1 Research?

Kit Components at a Glance

• Human COL3A1 microplate (pre-coated with capture antibody)
• Human COL3A1 standard
• Human COL3A1 detection antibody (HRP-conjugated)
• Chromogen solution
• Standard diluent, assay buffer, HRP substrate, stop solution, wash buffer, plate covers

🔬 Which research field does your work belong to?
Liver Fibrosis | Cancer & Tumor Stroma | Pulmonary Fibrosis | Cardiac Fibrosis | vEDS Research | Other
Share your application with us — we'd love to hear how you're using this kit in your studies!

📌 Reference Link: Human Collagen Type III Alpha 1 (COL3A1) ELISA Kit — KTE62513