Human Fibrinogen (FB) ELISA Kit — Product Review & Buying Guide for Coagulation and Inflammation Researchers

Fibrinogen: The 340-kDa Clotting Protein That Doubles as an Inflammatory Biomarker

Fibrinogen (FB, Factor I) is a 340-kDa soluble plasma glycoprotein synthesized in the liver that serves as the final substrate of the coagulation cascade. When thrombin cleaves fibrinogen into fibrin monomers, those monomers polymerize into the insoluble fibrin mesh that forms the structural backbone of every blood clot. But fibrinogen is not just a clotting factor — it is also an acute-phase protein, elevated in chronic inflammation, and a well-established independent risk factor for cardiovascular disease.

This dual identity makes fibrinogen a uniquely valuable biomarker. Elevated fibrinogen levels predict thrombotic risk, atherosclerosis, and cardiovascular events. In ulcerative colitis, fibrinogen, FDP, and D-dimer levels are potential biomarkers for disease severity. In chronic inflammation, fibrinogen is elevated alongside CRP, reflecting the shared inflammatory pathways that drive both coagulation and vascular risk.

The Human Fibrinogen (FB) ELISA Kit (KTE62515) from Abbkine offers a quantitative, sandwich ELISA solution for measuring this critical coagulation protein.

What's in the Box?

A complete, ready-to-use kit.

Advantages

1. Quantitative, Sandwich ELISA Format

The kit employs a two-site sandwich ELISA to quantitate fibrinogen in samples, providing quantitative, specific detection from a standard curve.

2. Broad Sample Compatibility

Validated for serum, plasma, cell culture supernatants, and other biological fluids — versatile for clinical, translational, and in vitro studies.

3. Dual-Readout Biological Relevance

Fibrinogen serves as both a coagulation factor and an inflammatory biomarker, making it valuable in multiple research contexts — from thrombosis to chronic inflammation to cardiovascular risk assessment.

4. Established Clinical Utility

Fibrinogen is a major risk factor for CVD with a direct link to inflammatory activity in atherosclerosis.

5. Complete Kit

All components included — no hidden costs or last-minute reagent sourcing.

6. Reliable Storage and Shipping

• Unopened kit: store at 2–8°C
• Shipped with gel pack and blue ice

Disadvantages

1. Human-Specific

This kit is specific to human fibrinogen — not validated for mouse, rat, or other species.

2. Research Use Only

Not intended for human or clinical diagnosis.

3. Assay Duration

Standard sandwich ELISA with a working time of 3–5 hours.

Who Should Buy This Kit?

✅ Buy this kit if you:

• Study coagulation, thrombosis, or hemostasis
• Research cardiovascular disease, atherosclerosis, or vascular risk
• Investigate chronic inflammation (rheumatoid arthritis, IBD, chronic infections)
• Work on ulcerative colitis and disease severity biomarkers
• Study liver function (fibrinogen is synthesized in the liver)
• Need to quantify human fibrinogen in serum, plasma, or cell culture supernatants
• Value dual clinical relevance — coagulation and inflammation
• Want a complete, ready-to-use kit for high-throughput screening

❌ Consider alternatives if you:

• Need to detect mouse or rat fibrinogen — check Abbkine's other offerings
• Are working with clinical diagnostic samples
• Require a faster assay (<3 hours)

Quick Specs Summary

📌 Reference Link: Human Fibrinogen (FB) ELISA Kit — KTE62515