ERβ Polyclonal Antibody (ABP0087): The Specificity Solution for Estrogen Receptor Beta Research
The ERα/ERβ Cross-Reactivity Problem That's Been Skewing Your Data
Estrogen receptor beta (ERβ) is a critical nuclear receptor mediating estrogen signaling in diverse tissues—from prostate and breast to brain and bone. Yet researchers have faced a persistent, maddening hurdle: antibody cross-reactivity with ERα, which shares structural homology but has distinct biological roles[reference:0]. Low-specificity antibodies can lead to misleading conclusions, particularly in tissues co-expressing both receptors.
Abbkine's ERβ Polyclonal Antibody (ABP0087) was engineered to solve this exact problem through rigorous epitope mapping and validation[reference:1]. Produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human ERβ (around the non-phosphorylation site of S105), it exhibits minimal cross-reactivity with ERα—confirmed by Western blot and IHC on mixed tissue lysates[reference:2].
Validated Performance Across Four Core Applications
Beyond specificity, experimental versatility defines a high-quality antibody. ABP0087 delivers consistent results across WB, IHC, IF, and ELISA:
| Application | Performance |
|---|---|
| Western Blot | Detects endogenous ERβ at ~55 kDa in MCF-7 (low ERβ) and PC-3 (high ERβ) cells, with clear band resolution even in low-expression samples |
| IHC | Pre-adsorbed formulation reduces background noise, enabling precise localization in FFPE sections of breast or brain tissue |
| Immunofluorescence | Visualizes ERβ in hippocampal neurons for memory regulation studies |
| ELISA | Compatible with standard protocols for ERβ quantification |
Knockout-Validated Specificity: The Data You Can Trust
Reproducibility remains a cornerstone of scientific integrity, yet many commercial antibodies lack transparent validation data. Abbkine sets a benchmark: ABP0087 is backed by knockout cell line controls (ERβ-/- HEK293T) to confirm target specificity[reference:3]. Batch-to-batch consistency is ensured through strict quality control, with each lot tested for titer and reactivity[reference:4].
Head-to-Head: ERβ Antibody Comparison
| Feature | ABP0087 | Generic Competitors |
|---|---|---|
| ERα Cross-reactivity | Minimal (validated) | Often significant |
| Knockout Validation | Yes | Rarely available |
| Applications | WB, IHC-P, IF, ELISA | Variable |
| Host | Rabbit | Variable |
| Reactivity | Human | Variable |
The Bottom Line
For researchers studying ERβ's unique role in prostate cancer, neuronal differentiation, or drug discovery, such specificity is non-negotiable. ABP0087 delivers the validated performance your estrogen signaling research demands.
📎 Reference: ERβ Polyclonal Antibody (ABP0087)
