EliKine™ Human CD54 ELISA Kit — Comprehensive Review & Buying Guide

EliKine™ Human CD54 (sICAM-1) ELISA Kit: Is It Right for Your Lab?

CD54, also known as ICAM-1 (Intercellular Adhesion Molecule 1) , is a transmembrane protein of the immunoglobulin superfamily. It plays a central role in immune cell adhesion, leukocyte extravasation, and inflammatory responses.[reference:5] Soluble ICAM-1 (sICAM-1/CD54) is a well-established biomarker for inflammation, cardiovascular disease, and autoimmune disorders.

The EliKine™ Human CD54 (sICAM-1) ELISA Kit (KTE6003) from Abbkine offers a sensitive, specific, and complete solution for quantifying this important adhesion molecule. Here's our detailed review.[reference:6]

What's in the Box?

A complete, ready-to-use kit.[reference:7]

Advantages

1. Exceptional Sensitivity

This kit delivers an industry-leading limit of detection of just 7.8 pg/mL with a calibration range of 7.8–500 pg/mL.[reference:8] For researchers working with low-abundance sICAM-1 samples, this sensitivity is a game-changer.

2. Outstanding Specificity

No significant cross-reactivity or interference between human sICAM-1 and analogues. Your data reflects true sICAM-1 levels — not background noise from related adhesion molecules.[reference:9]

3. Broad Sample Compatibility

Validated for serum, plasma, cell culture supernatants, and other biological fluids[reference:10] — adaptable to most experimental designs.

4. Published Track Record

This kit has been cited in 1 publication[reference:11] — real-world validation from the research community.

5. Direct HRP Detection System

The HRP-conjugated detection antibody format eliminates the need for secondary antibody steps — simplifying the workflow and reducing assay time.[reference:12]

6. Complete Kit

All necessary components — microplate, standards, diluent, assay buffer, HRP-conjugate, substrate, stop solution, wash buffer, and plate covers — are included.[reference:13]

7. Reliable Storage and Shipping

• Unopened kit: store at 2–8°C[reference:14]
• Shipped with gel pack and blue ice[reference:15]

Disadvantages

1. Narrower Calibration Range

The 7.8–500 pg/mL range may require dilution for samples with very high sICAM-1 concentrations.

2. Human-Specific

This kit is specific to human CD54/sICAM-1 — not validated for mouse, rat, or other species.[reference:16]

3. Research Use Only

Not approved for human or clinical diagnosis.[reference:17]

4. Assay Duration

Standard sandwich ELISA with a working time of 3–5 hours[reference:18].

Who Should Buy This Kit?

✅ Buy this kit if you:

• Study inflammation, immunology, or cardiovascular disease
• Need to quantify human sICAM-1/CD54 in serum, plasma, or cell culture
• Require ultra-high sensitivity (7.8 pg/mL LOD)
• Value specificity — no cross-reactivity with related molecules
• Prefer a complete, ready-to-use kit
• Want a kit with published validation (1 citation)
• Appreciate the simplicity of direct HRP detection

❌ Consider alternatives if you:

• Need to detect mouse or rat ICAM-1
• Are working with samples containing very high sICAM-1 levels (>500 pg/mL)
• Require a faster assay (<3 hours)

Quick Specs Summary

📌 Reference Link: EliKine™ Human CD54 ELISA Kit — KTE6003